Serban Karina A, Petrache Irina
Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, National Jewish Health, University of Colorado Denver, Anschutz Medical Campus, Denver, CO, USA.
Methods Mol Biol. 2018;1809:379-394. doi: 10.1007/978-1-4939-8570-8_25.
Elastase and chronic cigarette smoke exposure animal models are commonly used to study lung morphologic and functional changes associated with emphysema-like airspace enlargement in various animal species. This chapter describes the rationale for using these two models to study mechanisms of COPD pathogenesis and provides protocols for their implementation. E-cigarettes are an emerging health concern and may also contribute to lung disease. Accordingly, approaches to study e-cigarette vapors are provided. This chapter also includes methods and tools necessary to assess lung morphologic and functional changes in animals with emphysema-like airspace enlargement.
弹性蛋白酶和慢性香烟烟雾暴露动物模型常用于研究各种动物物种中与肺气肿样气腔扩大相关的肺部形态和功能变化。本章描述了使用这两种模型研究慢性阻塞性肺疾病（COPD）发病机制的原理，并提供了实施这些模型的方案。电子烟是一个新出现的健康问题，也可能导致肺部疾病。因此，本章还提供了研究电子烟烟雾的方法。本章还包括评估患有肺气肿样气腔扩大的动物肺部形态和功能变化所需的方法和工具。
