Figueiredo Israel, Vericimo Mauricio Afonso, Clemente Sergio Carmona São, Teixeira Gerlinde Agate Platais Brasil
Departamento Materno-Infantil, Faculdade de Medicina, Hospital Universitário Antonio Pedro, Universidade Federal Fluminense, Av. Marques do Paraná, 303, CEP: 24030-210 Centro, Niterói, Rio de Janeiro, Brazil.
Departamento de Imunobiologia, Instituto de Biologia, Universidade Federal Fluminense, Brazil.
Parasite Epidemiol Control. 2016 May 24;1(2):144-148. doi: 10.1016/j.parepi.2016.05.004. eCollection 2016 Jun.
Immunoreactivity to spp. is believed to be associated with frequency of fish intake. The objective of this study was to evaluate, using principal component analysis, the main factors potentially involved in reactivity to these nematodes in postpartum women.
Retrospective study conducted on a database of 309 postpartum women. All completed a structured questionnaire and had blood samples collected for ELISA analysis of specific immunoglobulins against total spp. antigens and assessment of reactivity. Parametric and nonparametric tests were used to assess factors for sensitization in the reactive and nonreactive groups, and a principal component analysis was performed. A Pearson correlation matrix with varimax rotation was used to assess the variables of interest (place of residence, age, number of prenatal visits, type of birth facility, fish intake and frequency, raw fish intake, fish handling, history of allergies).
After exclusions, samples from 203 women were assessed. Of these, 52 (25.6%) were reactive for anti- IgG. Most women claimed not to handle fish (n = 121) and eat fish only sporadically (n = 71). Significant differences in age were seen between the reactive and nonreactive groups (p = 0.001). The first two components explained 32.55% and 38.94% of variances in the nonreactive and reactive groups respectively. The adjusted matrix assigned greater probabilistic weight to weekly intake frequency (0.804), followed by raw fish intake (0.759), with differences in relation to the nonreactive group.
Correlation matrices revealed a direct relationship between seroreactivity to spp. and frequency of fish intake in a sample of postpartum women.
对 spp. 的免疫反应性被认为与鱼类摄入量有关。本研究的目的是使用主成分分析评估产后女性对这些线虫反应性的潜在主要因素。
对309名产后女性的数据库进行回顾性研究。所有人都完成了一份结构化问卷，并采集了血样用于针对总 spp. 抗原的特异性免疫球蛋白的ELISA分析和反应性评估。使用参数和非参数检验评估反应组和非反应组中的致敏因素，并进行主成分分析。使用具有方差最大化旋转的Pearson相关矩阵评估感兴趣的变量（居住地、年龄、产前检查次数、分娩机构类型、鱼类摄入量和频率、生鱼摄入量、鱼类处理方式、过敏史）。
排除后，对203名女性的样本进行了评估。其中，52人（25.6%）抗 - IgG呈反应性。大多数女性声称不处理鱼类（n = 121），仅偶尔食用鱼类（n = 71）。反应组和非反应组之间年龄存在显著差异（p = 0.001）。前两个成分分别解释了非反应组和反应组中32.55%和38.94%的方差。调整后的矩阵赋予每周摄入频率更大的概率权重（0.804），其次是生鱼摄入量（0.759），与非反应组存在差异。
相关矩阵显示产后女性样本中对 spp. 的血清反应性与鱼类摄入频率之间存在直接关系。
