Raz A, Zöller M
Exp Cell Res. 1986 Jan;162(1):127-41. doi: 10.1016/0014-4827(86)90431-3.
The pattern of cell substrate interaction, the cell surface composition and the organization of cytoskeletal elements was studied in tumour cell variants of the BSp73 rat adenocarcinoma displaying different metastatic capabilities and cell configuration. The non-metastasizing AS variant cells adhered to the substrate and spread via vinculin-containing focal contacts. These cells also synthesized, secreted and assembled fibronectin at the pericellular area. The metastasizing ASML variant cells adhered to the substrate at a slower rate via thick cytoplasmic protrusions, but were removed from the substrate by trypsin-EDTA slower than the non-metastasizing AS variant cells. The ASML cells also synthesized very low levels of both vinculin and fibronectin, displayed a diffuse pattern of actin and tubulin organization, and were unable to spread on the substrate. Spreading could not be induced in the ASML cells by seeding the cells on an extracellular matrix derived from bovine corneal endothelial cells or on concanavalin A (conA)-coated substrates, or by the addition of db-cAMP to the medium. The metastasizing cells expressed a unique and abundant cell surface glycoprotein of Mr 170 000 which was also shed into the growth medium. The relationships among the adhesive properties, the organization of cell surface components and of the cytoskeleton in the tumour cell variants, and the expression of their metastatic phenotype is discussed.
在具有不同转移能力和细胞形态的BSp73大鼠腺癌肿瘤细胞变体中，研究了细胞与底物的相互作用模式、细胞表面组成以及细胞骨架成分的组织情况。非转移型AS变体细胞通过含纽蛋白的粘着斑粘附于底物并铺展。这些细胞还在细胞周围区域合成、分泌并组装纤连蛋白。转移型ASML变体细胞通过粗大的细胞质突起以较慢的速率粘附于底物，但用胰蛋白酶 - 乙二胺四乙酸（EDTA）从底物上移除的速度比非转移型AS变体细胞慢。ASML细胞合成的纽蛋白和纤连蛋白水平也非常低，肌动蛋白和微管蛋白呈现弥散性组织模式，并且无法在底物上铺展。将细胞接种于源自牛角膜内皮细胞的细胞外基质上、伴刀豆球蛋白A（ConA）包被的底物上，或向培养基中添加二丁酰环磷腺苷（db - cAMP），均无法诱导ASML细胞铺展。转移型细胞表达一种独特且丰富的分子量为17万的细胞表面糖蛋白，该糖蛋白也会脱落到生长培养基中。本文讨论了肿瘤细胞变体中粘附特性、细胞表面成分和细胞骨架组织之间的关系，以及它们转移表型的表达。
