Shah Syed Nasar, Ahmad Mushtaq, Zafar Muhammad, Razzaq Abdul, Malik Khafsa, Rashid Neelam, Ullah Fazal, Iqbal Majid, Zaman Wajid
Department of Plant Sciences, Quaid- i- Azam University Islamabad, 45320, Pakistan.
Center of Plant Biodiversity, University of Peshawar, Peshawar, Pakistan.
Microsc Res Tech. 2018 Aug;81(8):902-913. doi: 10.1002/jemt.23055. Epub 2018 Jul 10.
For the robust identification of taxonomically complex fern family like Athyriaceae, light and scanning electron microscopy is significance implications. This article present first microscopic investigation of foliar micromorphology of 3 genera and 10 species belonging to Athyriaceae namely, Athyrium, Deparia, and Diplazium were collected from different localities in Malakand Division, Northern Pakistan. In present study we compare foliar micromorphology of all 10 species using standard protocols of light microcopy (LM) and scanning electron microscopy. Qualitative micromorphological variations in shape of epidermal cells, anticlinal wall pattern, stomatal type and shape, stomatal pore shape, guard cells shape, and trichomes types were studied. In addition, some quantitative characters were also studied and data were statistically analyzed in epidermal cell size, stomatal size, stomatal pore size, stomatal density, and stomatal index. The pivotal result of study include; shape of epidermal cell in all species is irregular on both abaxial and adaxial surfaces. The anticlinal walls are sinuous in most of the species but some species have irregular lobed and broadly lobed wall. Leaves are hypostomatic in all studied species. Two main categories of stomatal type were found: polocytic and anomocytic. Unicellular nonglandular trichomes were observed in only one species Athyrium mackinnoni. The variation in foliar micromorphological characters between the genera and within the species was useful in identification and classification and have potential taxonomic significance for species differentiation. An identification key using micromorphological characters are provided to distinguish genera and species.
对于像蹄盖蕨科这样分类复杂的蕨类植物科的可靠鉴定，光学显微镜和扫描电子显微镜具有重要意义。本文首次对蹄盖蕨科3个属10个物种的叶微观形态进行了研究，这些物种分别是蹄盖蕨属、介蕨属和双盖蕨属，采自巴基斯坦北部马拉坎德地区的不同地点。在本研究中，我们使用光学显微镜（LM）和扫描电子显微镜的标准方法比较了所有10个物种的叶微观形态。研究了表皮细胞形状、垂周壁模式、气孔类型和形状、气孔孔形状、保卫细胞形状以及毛状体类型的定性微观形态变化。此外，还研究了一些定量特征，并对表皮细胞大小、气孔大小、气孔孔大小、气孔密度和气孔指数的数据进行了统计分析。该研究的关键结果包括：所有物种的叶表皮细胞在背面和正面均不规则。大多数物种的垂周壁呈波状，但有些物种具有不规则的叶状和宽叶状壁。所有研究物种的叶均为下气孔型。发现了两种主要的气孔类型：多细胞型和不规则型。仅在一种麦氏蹄盖蕨中观察到单细胞非腺毛。属间和种内叶微观形态特征的变化有助于鉴定和分类，对物种分化具有潜在的分类学意义。提供了一个利用微观形态特征的鉴定检索表，以区分属和种。
