College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, China.
College of Life Science, Luoyang Normal University, Luoyang, China.
J Appl Microbiol. 2018 Nov;125(5):1526-1533. doi: 10.1111/jam.14044. Epub 2018 Aug 22.
Further investigations of the novel biological functions of SodA in Salmonella enterica serovar typhimurium (Salm. typhimurium).
A deletion mutant of sodA in Salm. typhimurium was constructed and its biological characteristics were analysed. More specifically, the roles of superoxide dismutase (SOD) in resistance to environmental stresses and serum, biofilm formation, epithelial cell adherence and invasion were investigated. Inactivation of sodA gene resulted in a slightly reduced growth rate, low SOD activity and increased susceptibility to reactive oxygen species and chicken serum. However, SodA was not involved in the motility of Salm. typhimurium. In addition, the sodA mutant exhibited a decreased capacity to form biofilm as well as to adhere and invade to HeLa cells, compared to the wild-type and complemented strains.
Salmonella typhimurium SOD appears to play an important role in resistance to serum and oxidative stress, biofilm formation, adherence to and invasion of epithelial cells.
This study illustrates the novel roles of SodA in serum resistance and biofilm formation in Salm. typhimurium, which may provide a candidate for the biofilm eradication and prevention of infections.
进一步研究新型 SodA 在鼠伤寒沙门氏菌（Salm. typhimurium）中的生物学功能。
构建了鼠伤寒沙门氏菌 sodA 的缺失突变体，并分析了其生物学特性。更具体地说，研究了超氧化物歧化酶（SOD）在抵抗环境应激和血清、生物膜形成、上皮细胞黏附和侵袭中的作用。 sodA 基因失活导致生长速度略有降低、SOD 活性降低、对活性氧和鸡血清的敏感性增加。然而， SodA 不参与鼠伤寒沙门氏菌的运动。此外，与野生型和互补菌株相比， sodA 突变体形成生物膜的能力以及黏附和侵袭 HeLa 细胞的能力降低。
鼠伤寒沙门氏菌 SOD 似乎在抵抗血清和氧化应激、生物膜形成、黏附和侵袭上皮细胞方面发挥重要作用。
本研究说明了 SodA 在鼠伤寒沙门氏菌血清抗性和生物膜形成中的新作用，这可能为生物膜清除和感染预防提供了一个候选目标。
