Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China. Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, People's Republic of China. Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, Tianjin, 300072, People's Republic of China.
Nanotechnology. 2018 Sep 28;29(39):395101. doi: 10.1088/1361-6528/aad257. Epub 2018 Jul 10.
The use of implants or indwelling medical devices has greatly enhanced the quality and efficacy of health care. However, foreign-body reactions (FBRs) and infections can lead to potential failure or removal of the devices, or increased morbidity and mortality of patients. Herein, we develop a silver nanoparticle (AgNP) loaded poly(hydroxyethyl methacrylate) hydrogel with spherical, interconnected 40 μm pores. The resulting hydrogels displayed good antibacterial properties regarding both gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Escherichia coli (E. coli)) in vitro and were highly efficient at inhibiting bacterial cell growth. Moreover, they exhibited an in vivo resistance to FBRs by reducing the immune responses, and completely prevented the formation of collagen capsules. Finally, in vivo studies of the E. coli infected mouse model demonstrated that the AgNP loaded porous hydrogels were highly efficient at resisting the bacterial FBRs and infections, while they promoted cell mitigation and infiltration. Findings from this work suggest that AgNP loaded porous hydrogels hold promise in various biomedical applications including in the new generation of implantable biomedical devices and tissue engineering scaffolds.
植入物或留置医疗器械的使用极大地提高了医疗保健的质量和效果。然而，异物反应（FBR）和感染会导致器械潜在失效或移除，或增加患者的发病率和死亡率。在此，我们开发了一种负载银纳米颗粒（AgNP）的具有球形、互连 40μm 孔的聚（羟乙基甲基丙烯酸酯）水凝胶。所得水凝胶在体外对革兰氏阳性菌（金黄色葡萄球菌）和革兰氏阴性菌（大肠杆菌（E. coli））均具有良好的抗菌性能，对细菌细胞生长具有高效抑制作用。此外，它们通过减少免疫反应表现出对 FBR 的体内抗性，并完全阻止了胶原胶囊的形成。最后，大肠杆菌感染小鼠模型的体内研究表明，负载 AgNP 的多孔水凝胶在抵抗细菌 FBR 和感染方面非常有效，同时促进了细胞缓解和浸润。这项工作的结果表明，负载 AgNP 的多孔水凝胶在各种生物医学应用中具有广阔的应用前景，包括新一代可植入生物医学设备和组织工程支架。
