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转录组测序和分子标记在血蛤性腺中的发现。

Transcriptome sequencing and molecular markers discovery in the gonads of Portunus sanguinolentus.

机构信息

Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China.

STU‑UNIVPM Joint Algal Research Center, Shantou University, Shantou 515063, China.

出版信息

Sci Data. 2018 Jul 10;5:180131. doi: 10.1038/sdata.2018.131.

Abstract

Crab culture has gained prominence in the last decade due to the large global market demand for live crabs and crab products. Portunus sanguinolentus is one of the economically important crab species in the Indo-Pacific region, with distinct differences in growth and size between male and female crabs, thus, leading to huge difference in their market values. The culture of P. sanguinolentus is still in its infancy, with crab supplies heavily dependent on wild catch. In order to unravel the molecular differences between male and female crabs, we generated a comprehensive transcriptomic dataset for P. sanguinolentus by sequencing the gonads of both sexes using the Illumina Hiseq 2500 system. Transcriptomes were assembled using Trinity de novo assembly followed by annotation. This transcriptomic data set for P. sanguinolentus would serve as an important reference data for genomic and genetic studies in this crab and related species.

摘要

由于全球对活蟹和蟹类产品的巨大市场需求,蟹类文化在过去十年中变得突出。血蟹是印度太平洋地区具有经济重要性的蟹类之一,其雌雄蟹之间的生长和体型存在明显差异,因此导致其市场价值存在巨大差异。血蟹的养殖仍处于起步阶段,蟹类供应严重依赖野生捕捞。为了揭示雌雄蟹之间的分子差异,我们使用 Illumina Hiseq 2500 系统对雌雄性腺进行测序,生成了血蟹的综合转录组数据集。使用 Trinity de novo 组装对转录组进行组装,然后进行注释。这个血蟹转录组数据集将成为该蟹及其相关物种基因组和遗传研究的重要参考数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2f15/6038849/d7c830c4f17c/sdata2018131-f1.jpg

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