CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, China.
South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, China.
PLoS One. 2018 Dec 5;13(12):e0207771. doi: 10.1371/journal.pone.0207771. eCollection 2018.
Soil salinization erodes the farmlands and poses a serious threat to human life, reuse of the saline-alkali lands as cultivated resources becomes increasingly prominent. Pacific white shrimp (Litopenaeus vannamei) is an important farmed aquatic species for the development and utilization of the saline-alkali areas. However, little is known about the adaptation mechanism of this species in terms of high-pH stress. In the present study, a transcriptome analysis on the gill tissues of L. vannamei in response to high-pH stress (pH 9.3 ± 0.1) was conducted. After analyzing, the cyclic nucleotide gated channel-Ca2+ (CNGC-Ca2+) and patched 1 (Ptc1) were detected as the majority annotated components in the cAMP signaling pathway (KO04024), indicating that the CNGC-Ca2+ and Ptc1 might be the candidate components for transducing and maintaining the high-pH stress signals, respectively. The immunoglobulin superfamily (IgSF), heat shock protein (HSP), glutathione s-transferase (GST), prophenoloxidase/phenoloxidase (proPO/PO), superoxide dismutase (SOD), anti-lipopolysaccharide factor (ALF) and lipoprotein were discovered as the major transcribed immune factors in response to high-pH stress. To further detect hub regulation-genes, protein-protein interaction (PPI) networks were constructed; the genes/proteins "Polymerase (RNA) II (DNA directed) polypeptide A" (POLR2A), "Histone acetyltransferase p300" (EP300) and "Heat shock 70kDa protein 8" (HSPA8) were suggested as the top three hub regulation-genes in response to acute high-pH stress; the genes/proteins "Heat shock 70kDa protein 4" (HSPA4), "FBJ murine osteosarcoma viral oncogene homolog" (FOS) and "Nucleoporin 54kDa" (NUP54) were proposed as the top three hub regulation-genes involved in adapting endurance high-pH stress; the protein-interactions of "EP300-HSPA8" and "HSPA4-NUP54" were detected as the most important biological interactions in response to the high-pH stress; and the HSP70 family genes might play essential roles in the adaptation of the high-pH stress environment in L. vannamei. These findings provide the first insight into the molecular and immune basis of L. vannamei in terms of high-pH environments, and the construction of a PPI network might improve our understanding in revealing the hub regulation-genes in response to abiotic stress in shrimp species and might be beneficial for further studies.
土壤盐渍化侵蚀农田，对人类生命构成严重威胁，因此将盐碱地重新用作耕地资源变得日益重要。凡纳滨对虾（Litopenaeus vannamei）是开发利用盐碱地的重要养殖水产物种。然而，目前对于该物种在高 pH 胁迫下的适应机制知之甚少。本研究采用高通量转录组测序技术分析了凡纳滨对虾鳃组织在高 pH 胁迫（pH 9.3 ± 0.1）下的转录组变化。分析结果表明，环核苷酸门控通道-Ca2+（CNGC-Ca2+）和 patched 1（Ptc1）被鉴定为 cAMP 信号通路（KO04024）的主要注释成分，提示 CNGC-Ca2+和 Ptc1可能分别作为高 pH 胁迫信号的转导和维持的候选成分。免疫球蛋白超家族（IgSF）、热休克蛋白（HSP）、谷胱甘肽 S-转移酶（GST）、原酚氧化酶/酚氧化酶（proPO/PO）、超氧化物歧化酶（SOD）、抗脂多糖因子（ALF）和脂蛋白被发现是对高 pH 胁迫反应的主要转录免疫因子。为了进一步检测关键调控基因，构建了蛋白质-蛋白质相互作用（PPI）网络；推测“聚合酶（RNA）II（DNA 指导）多肽 A”（POLR2A）、“组蛋白乙酰转移酶 p300”（EP300）和“热休克 70kDa 蛋白 8”（HSPA8）这 3 个基因/蛋白可能是急性高 pH 胁迫反应的关键调控基因；“热休克 70kDa 蛋白 4”（HSPA4）、“FBJ 鼠骨肉瘤病毒癌基因同源物”（FOS）和“核孔蛋白 54kDa”（NUP54）这 3 个基因/蛋白可能是适应慢性高 pH 胁迫的关键调控基因；检测到“EP300-HSPA8”和“HSPA4-NUP54”的蛋白相互作用是高 pH 胁迫反应的最重要的生物学相互作用；HSP70 家族基因可能在凡纳滨对虾适应高 pH 环境中发挥重要作用。这些发现为凡纳滨对虾在高 pH 环境下的分子和免疫基础提供了新的见解，构建 PPI 网络可能有助于深入了解虾类应对非生物胁迫的关键调控基因，并可能有助于进一步的研究。
