Garcia L A, Ordonez G A
Transfusion. 1976 Jan-Feb;16(1):32-41. doi: 10.1046/j.1537-2995.1976.16176130834.x.
Pluronic F-38 was used a precipitant of plasma proteins under varying conditions of pH and polymer concentration. Results indicated that marked differences in the solubility of the plasma proteins in F-38 solutions can be appled to the separation of plasma components. The feasibility of the industrial application of this fractionation method was tested in several experiments. Conditions were established for the preparation of albumin, human plasma protein fraction (HPPF), and immune serum globulin (ISG) with similar yield and purity as those prepared by the Cohn methods. Current procedures for the preparation of antihemophilic A (Facor VIII) concentrate and prothrombin complex (Factors II, VII, IX, and X) were adapted to the F-38 process by removal of the clotting factors from the starting plasma prior to polymer precipitation. In addition, a plasma protein solution free of lipoproteins, isoagglutinins, and clotting factors was developed which has proven useful as a perfusion medium in organ preservation.
普朗尼克F - 38在不同的pH值和聚合物浓度条件下被用作血浆蛋白的沉淀剂。结果表明，血浆蛋白在F - 38溶液中的溶解度存在显著差异，可用于血浆成分的分离。该分级分离方法在多个实验中测试了其工业应用的可行性。建立了制备白蛋白、人血浆蛋白组分（HPPF）和免疫血清球蛋白（ISG）的条件，其产量和纯度与用科恩方法制备的相似。通过在聚合物沉淀之前从起始血浆中去除凝血因子，将目前制备抗血友病A（因子VIII）浓缩物和凝血酶原复合物（因子II、VII、IX和X）的程序调整为适用于F - 38工艺。此外，还开发了一种不含脂蛋白、同种凝集素和凝血因子的血浆蛋白溶液，已证明其作为器官保存中的灌注介质很有用。
