Wickerhauser M
Transfusion. 1976 Jul-Aug;16(4):345-50. doi: 10.1046/j.1537-2995.1976.16476247055.x.
Four large-scale batches of Antihemophilic Factor (AHF, factor VIII) were prepared from plasma derived from 4 to 6-day-old blood applying a method developed for preparation of AHF from fresh frozen plasma. The AHF product was 6 to 9-fold concentrated over plasma with 7 to 10-fold purification and a recovery of 100 to 140 factor VIII units per liter of starting plasma. In terms of purity and yield, this is about half that of AHF obtained from fresh frozen plasma. The AHF concentrate was free of detectable thrombin and plasmin and the solubility of the dry product was comparable to that of the product derived from fresh plasma but the hemoglobin content was slightly increased. After further fractionation with polyethylene glycol (PEG 4000), a highly soluble AHF product 100-fold purified, and 30-fold concentrated, was obtained with 60% factor VIII recovery, which corresponds to a final yield of 60 to 85 factor VIII units per liter of starting plasma.
采用一种为从新鲜冷冻血浆制备抗血友病因子（AHF，凝血因子VIII）而开发的方法，从4至6日龄血液来源的血浆中制备了四批大规模的AHF。AHF产品相对于血浆浓缩了6至9倍，纯化了7至10倍，每升起始血浆中凝血因子VIII的回收率为100至140单位。就纯度和产量而言，这大约是从新鲜冷冻血浆中获得的AHF的一半。AHF浓缩物未检测到凝血酶和纤溶酶，干燥产品的溶解度与从新鲜血浆中获得的产品相当，但血红蛋白含量略有增加。用聚乙二醇（PEG 4000）进一步分级分离后，获得了一种高度可溶的AHF产品，其纯化了100倍，浓缩了30倍，凝血因子VIII回收率为60%，这相当于每升起始血浆的最终产量为60至85个凝血因子VIII单位。
