Guangzhou Center for Disease Control and Prevention, Guangzhou, Guangdong 510440, China.
Jiangsu Macro and Micro Test Med-tech Co., Limited., 101300, China.
Virus Res. 2018 Sep 2;256:201-208. doi: 10.1016/j.virusres.2018.07.005. Epub 2018 Jul 7.
To determine the evolutionary and phylodynamic history of DENV-1 in Guangdong, the strains detected between 1985 and 2015 were determined with phylogenetic and Bayesian analyses of the E gene. Three DENV-1 genotypes (I, V, and VI) were circulating in Guangdong, and genotype I was detected most frequently. The evolutionary rate of DENV-1 was estimated to be 1.03 × 10 nucleotide substitutions/site/year. The most recent ancestor of the viruses existed approximately 141 years ago. The observed epidemiological dynamics correlated with similar fluctuations in diversity, and the epidemiological dynamics of DENV-1 transmission reflect dramatic changes in the viral population sizes. Two recombination events were identified in those strains. The selection pressures were estimated and revealed an abundance of negatively selected sites but few positively selected sites. These data improve our understanding of the evolution and molecular epidemiology of DENV-1 and provide insights that will facilitate the surveillance and control of DENV-1.
为了确定广东地区登革热病毒 1 型（DENV-1）的进化和系统发育历史，我们对 1985 年至 2015 年间检测到的病毒株进行了 E 基因的系统发育和贝叶斯分析。在广东地区流行着三种 DENV-1 基因型（I、V 和 VI），其中基因型 I 最为常见。DENV-1 的进化率估计为 1.03×10 个核苷酸取代/位点/年。病毒的最近共同祖先大约存在于 141 年前。观察到的流行病学动态与多样性的相似波动相关，DENV-1 传播的流行病学动态反映了病毒种群规模的巨大变化。在这些病毒株中发现了两个重组事件。对选择压力进行了估计，结果表明存在大量的负选择位点，但正选择位点较少。这些数据提高了我们对 DENV-1 进化和分子流行病学的认识，并为 DENV-1 的监测和控制提供了有价值的信息。
