DFG Research Center for Regenerative Therapies Dresden, Faculty of Medicine, Technische Universität Dresden, Dresden, Germany.
Helmholtz Zentrum München, Institute for Diabetes and Obesity, Neuherberg, Germany.
Clin Immunol. 2018 Sep;194:87-91. doi: 10.1016/j.clim.2018.07.001. Epub 2018 Jul 7.
Type 1 diabetes is an autoimmune disease leading to insulin deficiency. Autoantibodies to beta cell proteins are already present in the asymptomatic phase of type 1 diabetes. Recent findings have suggested a number of additional minor autoantigens in patients with type 1 diabetes. We have established luciferase immunoprecipitation systems (LIPS) for anti-MTIF3, anti-PPIL2, anti-NUP50 and anti-MLH1 and analyzed samples from 500 patients with type 1 diabetes at onset of clinical disease and 200 healthy individuals who had a family history of type 1 diabetes but no evidence of beta cell autoantibodies. We show significantly higher frequencies of anti-MTIF3, anti-PPIL2 and anti-MLH1 in recent onset type 1 diabetes patients in comparison to controls. In addition, antibodies to NUP50 were associated with HLA-DRB103 and antibodies to MLH1 were associated with HLA-DRB104 genotypes.
1 型糖尿病是一种自身免疫性疾病，导致胰岛素缺乏。在 1 型糖尿病的无症状期，已经存在针对β细胞蛋白的自身抗体。最近的研究结果表明，1 型糖尿病患者存在许多其他次要自身抗原。我们已经建立了用于抗-MTIF3、抗-PPIL2、抗-NUP50 和抗-MLH1 的荧光素酶免疫沉淀系统（LIPS），并分析了 500 名临床疾病发病时的 1 型糖尿病患者和 200 名有 1 型糖尿病家族史但无β细胞自身抗体证据的健康个体的样本。与对照组相比，我们发现近期发病的 1 型糖尿病患者中抗-MTIF3、抗-PPIL2 和抗-MLH1 的频率明显更高。此外，NUP50 抗体与 HLA-DRB103 相关，而 MLH1 抗体与 HLA-DRB104 基因型相关。
