Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), College of Biotechnology and Pharmaceutical Engineering, School of Pharmacy, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Bioresour Technol. 2018 Oct;266:482-487. doi: 10.1016/j.biortech.2018.06.109. Epub 2018 Jul 2.
Fermentation wastewater (FW) and algal residue are major by-products of docosahexaenoic acid (DHA) fermentations utilizing Schizochytrium sp. In order to reduce production costs and environmental pollution, we explored the application of FW and algal-residue extract (AE) for DHA production. Components analysis showed that FW and AE contained some mineral elements and protein residues, respectively. When they were used for DHA fermentation, results showed that 20% replacement of fresh water by FW and 80% replacement of yeast extract nitrogen by AE reached DHA content of 22.23 g/L and 27.10 g/L, respectively. Furthermore, a novel medium that utilizes a mixture of FW and AE was applied for DHA fermentation, whereby the final DHA yield reached 28.45 g/L, 24.56% higher than conventional medium. The strategy of valorizing fermentation waste provides a new method for reducing the costs and reducing environmental pollution of microbial fermentations.
发酵废水（FW）和藻类残渣是利用裂殖壶菌生产二十二碳六烯酸（DHA）的主要副产物。为了降低生产成本和环境污染，我们探索了利用 FW 和藻渣提取物（AE）生产 DHA 的应用。成分分析表明，FW 和 AE 分别含有一些矿物质元素和蛋白质残留。当它们用于 DHA 发酵时，结果表明，用 FW 替代 20%的新鲜水，用 AE 替代 80%的酵母提取物氮，分别达到了 22.23 g/L 和 27.10 g/L 的 DHA 含量。此外，还应用了一种利用 FW 和 AE 混合物的新型培养基进行 DHA 发酵，最终 DHA 产量达到 28.45 g/L，比传统培养基提高了 24.56%。利用发酵废物的增值策略为降低微生物发酵的成本和减少环境污染提供了一种新方法。
