Development of an alternative medium via completely replaces the medium components by mixed wastewater and crude glycerol for efficient production of docosahexaenoic acid by Schizochytrium sp.

Exorbitant substrates for Schizochytrium culture result in the high cost of docosahexaenoic acid (DHA) production. In order to develop a feasible approach that is expected to reduce DHA production cost, Schizochytrium sp. S31 cultivation with a mixture of saline wastewater (SWW) and tofu whey wastewater (TWW) was investigated in this study. Using glucose as the carbon source, the maximum biomass and DHA yield in cultures using mixed wastewater containing 5% SWW reached 19.08 and 2.66 g/L, respectively, which were 2.29 and 2.66 times higher than those of cultures using control medium. Moreover, a good wastewater treatment performance was achieved as approximately 60% of the COD, TN, and TP were reduced in the cultures using mixed wastewater with a SWW ratio of 5%. The mixed wastewater presented better performance on DHA production than control medium using all tested carbon sources including glucose, fructose, and pure and crude glycerol. The components of control medium can be completely replaced by the mixed wastewater and crude glycerol. It is expected to effectively decrease the medium cost for DHA production and reduce the environmental risk of food processing wastewater.