1 Key Laboratory for the Genetics of Developmental, Neuropsychiatric Disorders (Ministry of Education), Bio-X Institutes, Shanghai Jiao Tong University, Shanghai, China.
2 The First People's Hospital of Chenzhou, Chenzhou, Hunan, China.
Cell Transplant. 2018 Aug;27(8):1195-1202. doi: 10.1177/0963689718784878. Epub 2018 Jul 11.
Pigs share many anatomical and physiological features with humans, offering a unique and viable model for biomedical research. Although porcine female germline stem cells (FGSCs) were identified in the juvenile ovary, no reports described the isolation and purification of FGSCs from the pig at sexual maturity. Here, we isolated, purified, and cultured FGSCs from porcine ovaries at sexual maturity. Furthermore, we established and characterized the porcine FGSC (pFGSC) lines. In addition, we found that pFGSC lines could differentiate into oocytes when injection into tissue grafts, including human ovarian tissues. The results show that FGSCs exist in ovaries of Banna mini-pigs at juvenile and sexually maturity. These findings have implications in animal biotechnology applications and regeneration medicine.
猪在解剖学和生理学特征上与人类有很多相似之处，为生物医学研究提供了独特而可行的模型。尽管已经在幼年猪的卵巢中发现了猪雌性生殖干细胞（FGSCs），但目前尚无报道描述从性成熟猪的卵巢中分离和纯化 FGSCs。在这里，我们从性成熟猪的卵巢中分离、纯化和培养了 FGSCs。此外，我们还建立并鉴定了猪 FGSC（pFGSC）系。此外，我们发现 pFGSC 系在注射到组织移植物中时可以分化为卵母细胞，包括人卵巢组织。这些结果表明，FGSCs 存在于版纳迷你猪的幼年和性成熟卵巢中。这些发现对于动物生物技术应用和再生医学具有重要意义。
