Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, China.
Institute of Electrical Engineering, Chinese Academy of Science, Beijing 100190, China.
Biomed Res Int. 2018 May 24;2018:7456898. doi: 10.1155/2018/7456898. eCollection 2018.
Renal dysfunction is one of the most common complications of liver cirrhosis and is associated with increased morbidity and mortality. However, no available technology can simultaneously support liver and renal function in these patients. The aim of this study was to evaluate the safety and efficacy of an artificial liver and renal support system in cynomolgus monkeys with surgery-induced ARF. The ARF model was established by ligature of bilateral renal arteries in eight cynomolgus monkeys, which were randomly divided into a treatment group ( = 4) and control group ( = 4). Biochemical indexes were determined before and after surgery. Blood endotoxin levels, biochemical indexes, and bacterial cultures were assessed at 0, 3, and 6 h during treatment. System pressures and vital signs were recorded at 1 h intervals. Pathological examination was performed after death. ARF was successfully established, based on significant elevation of biochemical indexes and pathological examination. The treatment group had significantly reduced biochemical indexes relative to the control group. Measurement of blood endotoxins and aerobic and anaerobic bacteria cultures indicated no bacterial growth. The system pressures and vital signs were stable during treatment. The results indicate that our support system for the treatment of cynomolgus monkeys with surgery-induced acute renal failure is safe and effective.
肾功能障碍是肝硬化最常见的并发症之一，与发病率和死亡率的增加有关。然而，目前尚无技术可以同时支持这些患者的肝功能和肾功能。本研究旨在评估人工肝和肾支持系统在手术诱导的急性肾衰竭的食蟹猴中的安全性和疗效。通过结扎 8 只食蟹猴的双侧肾动脉建立 ARF 模型，将其随机分为治疗组（n = 4）和对照组（n = 4）。手术前后测定生化指标。在治疗期间的 0、3 和 6 h 时评估血液内毒素水平、生化指标和细菌培养。每 1 h 记录系统压力和生命体征。死亡后进行病理检查。基于生化指标的显著升高和病理检查，成功建立了 ARF 模型。与对照组相比，治疗组的生化指标显著降低。血液内毒素和需氧及厌氧细菌培养的测量表明无细菌生长。治疗过程中系统压力和生命体征稳定。结果表明，我们用于治疗食蟹猴手术诱导的急性肾衰竭的支持系统是安全有效的。
