Šuškalo Nevena, Hasanagić Dino, Topalić-Trivunović Ljiljana, Kukrić Zoran, Samelak Ivan, Savić Aleksandar, Kukavica Biljana
University of Banja Luka, Faculty of Natural Sciences and Mathematics, Banja Luka, Bosnia and Herzegovina.
University of Banja Luka, Faculty of Technology, Banja Luka, Bosnia and Herzegovina.
Ecotoxicology. 2018 Oct;27(8):1095-1106. doi: 10.1007/s10646-018-1963-z. Epub 2018 Jul 10.
The complexity of ecological conditions in urban areas imposes the plant species need for the development of various biochemical and physiological adaptive strategies. The aim of our research was to examine the antioxidative and antifungal metabolism of species Pinus nigra, Picea omorika, Tilia cordata and Betula pendula from the area of Banja Luka City (urban area) during two vegetation seasons (spring and autumn) and compared with the same species from forest habitats. Changes in the protein concentration, activity and isoenzyme profiles of peroxidases (POD, EC 1.11.1.7), content and antioxidative activity of total phenols and antifungal activity in leaves and needles of the plants from the urban area and forest habitats were monitored. The obtained results indicate that urban areas induce changes in antioxidative metabolism in all examined species, but that the response is species specific. The most sensitive parameter that indicates different adaptation strategy of Pinus nigra, Picea omorika, Tilia cordata and Betula pendula to environment conditions in the urban area were peroxidase isoenzyme patterns. Less specific parameter was phenol content even though there are some indications for role of their antioxidative capacity in the adjustment to specific habitat. In addition, each species had different metabolic strategy to cope with the changes caused by the urban environment.
城市地区生态条件的复杂性使得植物物种需要发展各种生化和生理适应策略。我们研究的目的是考察巴尼亚卢卡市（市区）的黑松、塞尔维亚云杉、心叶椴和垂枝桦在两个植被季节（春季和秋季）的抗氧化和抗真菌代谢，并与来自森林栖息地的相同物种进行比较。监测了市区和森林栖息地植物叶片和针叶中过氧化物酶（POD，EC 1.11.1.7）的蛋白质浓度、活性和同工酶谱、总酚含量和抗氧化活性以及抗真菌活性的变化。所得结果表明，市区会引起所有受试物种抗氧化代谢的变化，但这种反应具有物种特异性。表明黑松、塞尔维亚云杉、心叶椴和垂枝桦对市区环境条件具有不同适应策略的最敏感参数是过氧化物酶同工酶模式。酚含量是不太具特异性的参数，尽管有一些迹象表明其抗氧化能力在适应特定生境中发挥作用。此外，每个物种应对城市环境引起的变化都有不同的代谢策略。
