el Daher N, Betts R F
J Infect Dis. 1985 Dec;152(6):1197-205. doi: 10.1093/infdis/152.6.1197.
With a standard chromium release assay, natural killing (NK) activity of peripheral mononuclear cells (PMCs) from 28 individuals was compared based on the ability of sera to support antibody-dependent cell-mediated cytotoxicity (ADCC) for cells infected with herpes simplex virus (HSV). PMCs from all 20 individuals whose sera produced ADCC were capable of killing HSV-infected cells compared with none of the PMCs from the eight individuals whose sera did not produce ADCC (mean specific release, 33.2% vs. 6.8%). Results could not be explained by contaminating serum in the assay or by ineffective NK by the PMCs from the eight negative subjects because many of them killed the k562 myeloid cell line as effectively as PMCs from other individuals. In addition, NK could be eliminated by preincubation of effector cells at 37 C, and the capacity to kill by PMCs could be reconstituted by incubation in serum. Killing was more a function of source of serum rather than source of cells.
采用标准的铬释放试验，基于血清支持针对单纯疱疹病毒（HSV）感染细胞的抗体依赖性细胞介导的细胞毒性（ADCC）的能力，比较了28名个体外周血单个核细胞（PMC）的自然杀伤（NK）活性。与血清未产生ADCC的8名个体的PMC均不能杀伤HSV感染细胞相比，血清产生ADCC的所有20名个体的PMC均能够杀伤HSV感染细胞（平均特异性释放率分别为33.2%和6.8%）。试验结果不能用试验中污染的血清或8名阴性受试者的PMC无效的NK来解释，因为他们中的许多人杀伤k562髓系细胞系的效果与其他个体的PMC一样有效。此外，效应细胞在37℃预孵育可消除NK，而PMC的杀伤能力可通过在血清中孵育来重建。杀伤更多地取决于血清来源而非细胞来源。
