Rappocciolo G, Toso J F, Torpey D J, Gupta P, Rinaldo C R
Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh, Pennsylvania 15261.
J Clin Microbiol. 1989 Jan;27(1):41-8. doi: 10.1128/jcm.27.1.41-48.1989.
Mononuclear leukocytes from human immunodeficiency virus (HIV)-seronegative and -seropositive homosexual men lysed HIV-infected U937 cells to a significantly greater degree than uninfected U937 cells. Depletion of cell subsets with monoclonal antibodies and complement indicated that the effector cells were primarily of the CD16+ phenotype. Acid-stable alpha interferon (IFN-alpha) production induced by the HIV-infected cells correlated with, although was not an absolute requisite for, preferential lysis of the infected targets. The activity of these CD16+, natural killer (NK) cells decreased in relation to the duration of HIV infection and the presence of acquired immunodeficiency syndrome. Pretreatment of peripheral blood mononuclear cells from HIV-seronegative subjects, but not HIV-seropositive men, with IFN-alpha or recombinant interleukin-2 enhanced lysis of both uninfected and HIV-infected U937 cells. These results suggest that IFN-alpha-associated, NK-like mechanisms are active in the cytotoxic response against HIV-infected cells and that HIV infection results in an early and progressive depression of such responses. Prospective investigations may be useful in determining the role of this NK cell response in the natural history and pathogenesis of HIV infection and the efficacy of therapeutic modalities.
来自人类免疫缺陷病毒（HIV）血清学阴性和血清学阳性同性恋男性的单核白细胞对HIV感染的U937细胞的裂解程度明显高于未感染的U937细胞。用单克隆抗体和补体清除细胞亚群表明，效应细胞主要为CD16 +表型。HIV感染细胞诱导的酸稳定α干扰素（IFN-α）产生与感染靶标的优先裂解相关，尽管不是绝对必需的。这些CD16 +自然杀伤（NK）细胞的活性随着HIV感染持续时间和获得性免疫缺陷综合征的存在而降低。用IFN-α或重组白细胞介素-2预处理HIV血清学阴性受试者而非HIV血清学阳性男性的外周血单核细胞，可增强对未感染和HIV感染的U937细胞的裂解。这些结果表明，与IFN-α相关的NK样机制在针对HIV感染细胞的细胞毒性反应中起作用，并且HIV感染导致这种反应的早期和进行性抑制。前瞻性研究可能有助于确定这种NK细胞反应在HIV感染的自然史和发病机制中的作用以及治疗方式的疗效。
