College of Pharmacy, Woosuk University, Jeonbuk, South Korea.
College of Pharmacy, Dankook University, Cheonan, South Korea.
J Pharm Pharmacol. 2018 Oct;70(10):1423-1429. doi: 10.1111/jphp.12979. Epub 2018 Jul 10.
This study was conducted to evaluate the longevity potential of damaurone D (DaD), a component of the damask rose, in the animal model Caenorhabditis elegans.
To investigate the effect of DaD on the longevity, lifespan assay was carried out. Fluorescence intensity of transgenic mutants was quantified to test the expression levels of stress proteins. A genetic study using single gene knockout mutants was designed to determine the target genes of DaD.
DaD prolonged the mean lifespan of wild-type nematodes by 16.7% under normal conditions and also improved their stress endurance under thermal, osmotic, and oxidative stress conditions. This longevity-promoting effect could be attributed to in vivo antioxidant capacity and its up-regulating effects on the expressions of stress-response proteins such as SOD-3 and HSP-16.2. In addition, DaD treatment attenuated food intake, body length, lipofuscin accumulation and age-dependent decline of motor ability. Gene-specific mutant studies showed the involvement of genes such as daf-2, age-1, and daf-16.
These results suggest that DaD has beneficial effects on the longevity, and thus it can be a valuable plant origin lead compound for the development of nutraceutical preparations targeting ageing and ageing-related diseases.
本研究旨在评估大马酮 D（DaD）作为大马士革玫瑰的一种成分在秀丽隐杆线虫动物模型中的长寿潜力。
为了研究 DaD 对长寿的影响，进行了寿命测定实验。通过定量测定转(transgenic)基因突变体的荧光强度来测试应激蛋白的表达水平。设计了一项使用单基因敲除突变体的遗传研究，以确定 DaD 的靶基因。
DaD 在正常条件下使野生型线虫的平均寿命延长了 16.7%，并提高了它们在热、渗透和氧化应激条件下的应激耐力。这种延长寿命的作用可能归因于体内抗氧化能力及其对 SOD-3 和 HSP-16.2 等应激反应蛋白表达的上调作用。此外，DaD 处理还减弱了摄食量、体长、脂褐素积累以及运动能力随年龄的下降。基因特异性突变体研究表明，daf-2、age-1 和 daf-16 等基因参与其中。
这些结果表明，DaD 对长寿有有益的影响，因此它可以成为一种有价值的植物来源的先导化合物，用于开发针对衰老和与衰老相关疾病的营养保健品。
