School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.
Food Funct. 2018 Oct 17;9(10):5273-5282. doi: 10.1039/c8fo01680a.
Blueberry is rich in bioactive phytochemicals with a wide of range of biological activities and health benefits. However, little is known about their effects on aging. The objectives of this study were to evaluate the effects of supplementation with a blueberry extract (BE) on lifespan and stress resistance using Caenorhabditis elegans (C. elegans) as a model. The mechanisms of these effects were explored using RNAi technology. The mean lifespan of C. elegans treated with BE at 50, 100, and 200 mg mL-1 was significantly increased by 22.2%, 36.5%, and 44.4%, respectively, in a dose-dependent manner. In addition, supplementation with BE improved motility and decreased lipofuscin accumulation. C. elegans pretreated with BE were more resistant than untreated C. elegans to stresses (heat, ultraviolet-B radiation, and paraquat). Treatment with BE resulted in up-regulation of genes related to antioxidant systems, including sod-3, cat-1, mev-1, skn-1, mek-1, nhr-8, and daf-16. Suppression of daf-16 by RNAi shortened the lifespan of C. elegans and inhibited the expression of sod-3, suggesting that BE may regulate sod-3 downstream of daf-16 to extend lifespan and stress resistance. Our findings revealed that, in C. elegans, BE can prolong the lifespan, improve health indexes, and enhance stress resistance.
蓝莓富含具有广泛生物活性和健康益处的生物活性植物化学物质。然而，关于它们对衰老的影响知之甚少。本研究的目的是评估蓝莓提取物（BE）对秀丽隐杆线虫（C. elegans）寿命和抗逆性的影响，将其作为模型。使用 RNAi 技术探索这些影响的机制。BE 在 50、100 和 200 mg mL-1 浓度下处理的 C. elegans 的平均寿命分别显著增加了 22.2%、36.5%和 44.4%，呈剂量依赖性。此外，BE 的补充提高了运动能力并减少了脂褐素的积累。与未经处理的 C. elegans 相比，用 BE 预处理的 C. elegans 对热、紫外线-B 辐射和百草枯等应激更具抗性。BE 的处理导致与抗氧化系统相关的基因上调，包括 sod-3、cat-1、mev-1、skn-1、mek-1、nhr-8 和 daf-16。通过 RNAi 抑制 daf-16 缩短了 C. elegans 的寿命并抑制了 sod-3 的表达，表明 BE 可能通过调节 daf-16 下游的 sod-3 来延长寿命和增强抗逆性。我们的研究结果表明，在 C. elegans 中，BE 可以延长寿命、改善健康指标并增强抗逆性。
