United States Department of Agriculture, Agricultural Research Service, Southern Regional Research Center, 1100 Robert E. Lee Boulevard, New Orleans, LA, 70124, USA.
Sci Rep. 2018 Jul 11;8(1):10458. doi: 10.1038/s41598-018-28773-4.
Cottonseeds are classified as glanded or glandless seeds depending on the presence or absence of gossypol glands. Glanded cottonseed has anticancer property and glandless cottonseed was reported to cause cancer in one animal study. It is important to investigate the effect of bioactive components from cottonseeds. Our objectives were to isolate ethanol extracts from cottonseeds and investigate their effects on human cancer cells. A protocol was developed for isolating bioactive extracts from seed coat and kernel of glanded and glandless cottonseeds. HPLC-MS analyzed the four ethanol extracts but only quercetin was identified in the glandless seed coat extract. Residual gossypol was detected in the glanded and glandless seed kernel extracts and but only in the glanded seed coat extract. Ethanol extracts were used to treat human cancer cells derived from breast and pancreas followed by MTT assay for cell viability. Ethanol extracts from glanded and glandless cottonseed kernels and gossypol significantly decreased breast cancer cell mitochondrial activity. Ethanol extract from glanded cottonseed kernel and gossypol also significantly decreased pancreas cancer cell mitochondrial activity. These results suggest that ethanol extracts from cottonseeds, like gossypol, contain anticancer activities.
棉籽根据是否存在棉酚腺而被分为有腺棉籽和无腺棉籽。有腺棉籽具有抗癌特性，而一项动物研究报告称无腺棉籽会致癌。因此，研究棉籽中生物活性成分的作用非常重要。我们的目的是从棉籽中分离出乙醇提取物，并研究它们对人类癌细胞的影响。我们制定了从有腺和无腺棉籽的种皮和种仁中分离生物活性提取物的方案。HPLC-MS 分析了四种乙醇提取物，但仅在无腺种皮提取物中鉴定出了槲皮素。在有腺和无腺棉籽仁提取物中均检测到了残留的棉酚，但仅在有腺种皮提取物中检测到了棉酚。用乙醇提取物处理源自乳腺和胰腺的人类癌细胞，然后通过 MTT 法测定细胞活力。有腺和无腺棉籽仁以及棉酚的乙醇提取物显著降低了乳腺癌细胞的线粒体活性。有腺棉籽仁乙醇提取物和棉酚也显著降低了胰腺癌细胞的线粒体活性。这些结果表明，棉籽的乙醇提取物（如棉酚）具有抗癌活性。
