牙源性角化囊肿中 Fra-1、c-Jun 和 c-Fos 的过表达：与增殖和抗凋亡活性的潜在相关性。

Overexpression of Fra-1, c-Jun and c-Fos in odontogenic keratocysts: potential correlation with proliferative and anti-apoptotic activity.

机构信息

The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Wuhan University, Wuhan, China.

出版信息

Histopathology. 2018 Dec;73(6):933-942. doi: 10.1111/his.13705. Epub 2018 Nov 4.

DOI:10.1111/his.13705

PMID:29993138

Abstract

AIMS

The purpose of this study was to explore the potential involvement of Fra-1, c-Jun and c-Fos, three vital members of the AP-1 complex, in the pathogenesis of odontogenic keratocysts (OKCs).

METHODS AND RESULTS

Tissue samples, containing 10 normal oral mucosa (OM), 10 dentigerous cysts (DC) and 32 OKC specimens, were applied to investigate the expression levels of Fra-1, c-Jun and c-Fos by immunohistochemistry and real-time-quantitative polymerase chain reaction (RT-qPCR). The association between Fra-1, c-Jun and c-Fos expression levels and markers of proliferation [Ki-67, proliferating cell nuclear antigen (PCNA)], anti-apoptosis (Bcl-2) was then investigated in the OKC serial tissue sections. The results showed that Fra-1, c-Jun and c-Fos expression levels were increased significantly in OKCs compared to these in OM and DC tissue samples. Meanwhile, the expression levels of Fra-1, c-Jun and c-Fos were associated positively with the expression levels of Ki-67, PCNA and Bcl-2, as confirmed further by double-labelling immunofluorescence analysis and hierarchical analysis.

CONCLUSIONS

This study revealed for the first time that Fra-1, c-Jun and c-Fos were overexpressed in OKCs and had a close correlation with proliferation and anti-apoptosis potential of OKCs.

摘要

目的

本研究旨在探讨 AP-1 复合物的三个重要成员 Fra-1、c-Jun 和 c-Fos 是否参与牙源性角化囊肿（OKC）的发病机制。

方法和结果

采用免疫组织化学和实时定量聚合酶链反应（RT-qPCR）方法，检测 10 例正常口腔黏膜（OM）、10 例含牙囊肿（DC）和 32 例 OKC 组织样本中 Fra-1、c-Jun 和 c-Fos 的表达水平。然后，在 OKC 连续组织切片中，研究 Fra-1、c-Jun 和 c-Fos 表达水平与增殖标志物（Ki-67、增殖细胞核抗原（PCNA））、抗凋亡（Bcl-2）之间的关系。结果表明，与 OM 和 DC 组织样本相比，OKC 中 Fra-1、c-Jun 和 c-Fos 的表达水平显著升高。同时，Fra-1、c-Jun 和 c-Fos 的表达水平与 Ki-67、PCNA 和 Bcl-2 的表达水平呈正相关，双标记免疫荧光分析和层次分析进一步证实了这一点。