de Sousa Suzana Orsini Machado, Mesquita Ricardo Alves, Pinto Décio Santos, Gutkind Silvio
Department of Oral Pathology, University of São Paulo, School of Dentistry, São Paulo, Brazil.
J Oral Pathol Med. 2002 Feb;31(2):78-81. doi: 10.1046/j.0904-2512.2001.10012.x.
Studies have addressed the relevance of c-Jun and c-Fos proteins in cancer development. In the present study, the expression of c-Jun and c-Fos, the major components of transcription factor activator protein (AP1), were evaluated to determine possible alterations to these factors in oral squamous cell carcinoma (OSCC).
Fifteen cases of normal oral mucosa and 20 cases of OSCC were retrieved from the Archives of the Surgical Pathology Service at the University of São Paulo. The samples of normal oral mucosa or OSCC originated from different oral mucosal sites. Tissues were submitted for immunohistochemical analysis to detect c-Jun and c-Fos proteins. The OSCC was classified as well, intermediate or poorly differentiated.
The results showed that both c-Jun and c-Fos are expressed in normal oral mucosa and in OSCC. In normal mucosa, immunoreactivity for c-Jun was detected in the cytoplasm of the upper basal layers, while in OSCC, c-Jun was detected in the nuclei of the cells. C-Fos expression was observed in the nuclei of cells, both in normal mucosa and in OSCC, but its expression varied according to the cell layer in normal mucosa, and the differentiation of OSCC.
The nuclear expression of c-Jun in OSCC, in contrast to its cytoplasmic expression in normal oral mucosa, indicates that c-Jun may have a role in the development of oral cancer. In contrast, the absence of both c-Jun and c-Fos in poorly differentiated carcinoma might be useful in understanding the cell cycle events important in uncontrolled cell growth.
已有研究探讨了c-Jun和c-Fos蛋白在癌症发展中的相关性。在本研究中，对转录因子激活蛋白（AP1）的主要成分c-Jun和c-Fos的表达进行评估，以确定口腔鳞状细胞癌（OSCC）中这些因子可能存在的改变。
从圣保罗大学外科病理科档案中选取15例正常口腔黏膜和20例OSCC病例。正常口腔黏膜或OSCC样本来自不同的口腔黏膜部位。将组织进行免疫组化分析以检测c-Jun和c-Fos蛋白。OSCC也被分为高分化、中分化或低分化。
结果显示，c-Jun和c-Fos在正常口腔黏膜和OSCC中均有表达。在正常黏膜中，c-Jun的免疫反应性在上层基底层细胞质中检测到，而在OSCC中，c-Jun在细胞核中检测到。在正常黏膜和OSCC的细胞核中均观察到c-Fos表达，但其表达在正常黏膜中根据细胞层不同而变化，在OSCC中则根据分化程度不同而变化。
与正常口腔黏膜中c-Jun的细胞质表达相反，其在OSCC中的核表达表明c-Jun可能在口腔癌发展中起作用。相比之下，低分化癌中c-Jun和c-Fos均缺失可能有助于理解在细胞无节制生长中重要的细胞周期事件。
