Soluk Tekkeşın Merva, Mutlu Sevcihan, Olgaç Vakur
Department of umor Pathology, İstanbul University, Institute of Oncology, İstanbul, Turkey.
Turk Patoloji Derg. 2012;28(1):49-55. doi: 10.5146/tjpath.2012.01097.
The aim of the study was to determine the apoptotic features and proliferation potential of odontogenic keratocysts compared with ameloblastomas and radicular cysts by analysing the role of bax, bcl-2, and Ki-67.
The study material consisted of 20 odontogenic keratocysts, 20 radicular cysts, and 20 ameloblastomas. Immunohistochemically, bax, bcl-2 and Ki-67 were applied. The positive cells were evaluated in both neoplastic/nonneoplastic odontogenic epithelium and connective tissue cells.
Ameloblastoma showed stronger bcl-2 expression than odontogenic keratocysts and radicular cysts. Bcl-2 expression in the whole thickness of epithelium and connective tissue of odontogenic keratocyst was significantly higher than radicular cyst. The expression of bax in the epithelium of radicular cyst was significantly higher than odontogenic keratocyst and ameloblastoma. The lining epithelium of odontogenic keratocyst showed stronger Ki-67 expression than ameloblastoma and radicular cyst.
The proliferation potential of the epithelium and the overexpression of various anti-apoptotic proteins in odontogenic epithelial tumors are quite significant for their clinical behaviour. High expressions of bcl-2 and Ki-67 in odontogenic keratocysts accord with their aggressive clinical behaviour and a high recurrence rate.
本研究旨在通过分析bax、bcl - 2和Ki - 67的作用，确定牙源性角化囊肿与成釉细胞瘤和根尖囊肿相比的凋亡特征和增殖潜能。
研究材料包括20例牙源性角化囊肿、20例根尖囊肿和20例成釉细胞瘤。采用免疫组织化学方法检测bax、bcl - 2和Ki - 67。对肿瘤性/非肿瘤性牙源性上皮和结缔组织细胞中的阳性细胞进行评估。
成釉细胞瘤的bcl - 2表达强于牙源性角化囊肿和根尖囊肿。牙源性角化囊肿上皮和结缔组织全层的bcl - 2表达明显高于根尖囊肿。根尖囊肿上皮中的bax表达明显高于牙源性角化囊肿和成釉细胞瘤。牙源性角化囊肿的衬里上皮Ki - 67表达强于成釉细胞瘤和根尖囊肿。
牙源性上皮肿瘤中上皮的增殖潜能和各种抗凋亡蛋白的过表达对其临床行为具有重要意义。牙源性角化囊肿中bcl - 2和Ki - 67的高表达与其侵袭性临床行为和高复发率相符。
