IEEE Trans Biomed Eng. 2019 Jan;66(1):23-29. doi: 10.1109/TBME.2018.2836949. Epub 2018 May 15.
A wireless, multichannel system for real-time measurement of the cellular-temperature response to drugs is developed.
The acquisition system applies a high-precision reference resistor and a low real-time measurement current (below or equal to 0.14 mA) to reduce self-heating via the intermittent measurement. Cells of a small volume cell medium are cultured on the surface of the platinum thermal resistor and subsequently measured in the incubator.
The resistance resolution of the circuit exhibits 20 mΩ, which corresponds to no more than 0.01 °C. The resistance deviations of each channel are corrected with software compensation. The linearity between the temperature and resistance of the sensors lies above 0.999 in the applied temperature range (30 °C-42 °C). Observations with the scanning electron microscope show that the cells grow well on the sensor surface. The latter is composed of a glass glaze, which is nontoxic for organisms. The cell population temperature measurements under norepinephrine action present an obvious temperature rise, which can be the result of the drug binding to the receptors on cell membrane thus promoting a cationic inflow.
The platinum sensor and multichannel acquisition system can be used to determine the temperature changes of cells in their original state.
The wireless, real-time, high-throughput temperature detection method is particularly suitable to evaluate the thermogenic ability of growing cells that interact with other matter or organisms. The proposed method can help to explore thermal changes in cell populations, intercellular connections, and social connections of cells.
开发一种用于实时测量细胞对药物的温度响应的无线多通道系统。
采集系统采用高精度参考电阻和低实时测量电流（低于或等于 0.14 mA），通过间歇测量来减少自热。小体积细胞培养基中的细胞被培养在铂热敏电阻的表面，然后在孵育箱中进行测量。
该电路的电阻分辨率为 20 mΩ，对应于不超过 0.01°C 的温度变化。每个通道的电阻偏差都通过软件补偿进行校正。传感器的温度和电阻之间的线性度在应用温度范围内（30°C-42°C）高于 0.999。扫描电子显微镜观察表明，细胞在传感器表面生长良好。传感器由玻璃釉料组成，对生物体无毒。去甲肾上腺素作用下的细胞群体温度测量显示出明显的温度升高，这可能是药物与细胞膜上的受体结合从而促进阳离子内流的结果。
铂传感器和多通道采集系统可用于确定细胞在原始状态下的温度变化。
无线、实时、高通量的温度检测方法特别适用于评估与其他物质或生物体相互作用的生长细胞的产热能力。该方法可以帮助探索细胞群体、细胞间连接和细胞的社会联系中的热变化。
