Kallioinen M
J Pathol. 1985 Oct;147(2):97-102. doi: 10.1002/path.1711470204.
Specific antisera to the human laminin P1 fragment and the 7S domain of type IV collagen were used to investigate the ultrastructural location of these main basement membrane (BM) components in a dermal cylindroma. Thick frozen sections were treated by the immunoperoxidase method, postfixed, embedded in epon and then sectioned for electron microscopic examination. Laminin and type IV collagen were detected in all layers of the cylindroma BM, together with non-specifically stained, diffusely distributed fibrillar structures, possible microfibrils and anchoring fibrils or residual stromal collagen fibres. The mixed presence of the BM components indicates that these substances codistribute rather than occurring as separate layers even when forming neoplastic basement membranes.
使用针对人层粘连蛋白P1片段和IV型胶原7S结构域的特异性抗血清，研究这些主要基底膜（BM）成分在皮肤圆柱瘤中的超微结构定位。厚冰冻切片采用免疫过氧化物酶法处理，后固定，包埋在环氧树脂中，然后切片进行电子显微镜检查。在圆柱瘤BM的所有层中均检测到层粘连蛋白和IV型胶原，同时还有非特异性染色、弥漫分布的纤维状结构，可能是微原纤维、锚定纤维或残留的基质胶原纤维。BM成分的混合存在表明，即使在形成肿瘤性基底膜时，这些物质也是共分布的，而不是形成单独的层。
