Pfaltz M, Bruckner-Tuderman L, Schnyder U W
Department of Dermatology, University Hospital, Zurich, Switzerland.
J Cutan Pathol. 1989 Dec;16(6):388-95. doi: 10.1111/j.1600-0560.1989.tb00591.x.
Cylindroma basement membranes (BMs) were studied by electron microscopy and immunofluorescence with antibodies to Types IV and VII collagen in order to correlate the ultrastructure with the biochemical characteristics. Type IV collagen served as a marker for lamina densa structures and Type VII collagen for the anchoring fibrils. Ultrastructurally, the cylindroma BMs were composed of a wide band of BM-like material in which numerous anchoring fibrils were embedded. The BMs surrounding the tumor cell clusters and nodules of BM-like material entrapped within the tumor cell islands stained positively for both Types IV and VII collagen. We conclude, that anchoring fibrils are a major component of cylindroma BM zone. A trichoepithelioma investigated as control showed a BM similar to the one found at the dermoepidermal junction, i.e. a BM distinctly different from the one of cylindroma. The trichoepithelioma BMs and nodules of BM-like material included within the tumor also stained positively for both Type IV and VII collagen.
为了将超微结构与生化特征相关联，利用电子显微镜以及针对IV型和VII型胶原蛋白的抗体进行免疫荧光，对圆柱瘤基底膜（BMs）展开了研究。IV型胶原蛋白作为致密板结构的标志物，VII型胶原蛋白作为锚定原纤维的标志物。在超微结构上，圆柱瘤BMs由一条宽带状的类基底膜物质构成，其中嵌入了众多锚定原纤维。围绕肿瘤细胞簇的基底膜以及困于肿瘤细胞岛内的类基底膜物质结节，IV型和VII型胶原蛋白染色均呈阳性。我们得出结论，锚定原纤维是圆柱瘤BM区域的主要成分。作为对照研究的毛发上皮瘤显示出与在真皮表皮交界处发现的基底膜相似，即与圆柱瘤的基底膜明显不同。毛发上皮瘤的基底膜以及肿瘤内包含的类基底膜物质结节，IV型和VII型胶原蛋白染色也均呈阳性。
