Univ. Estadual Paulista, Faculdade de Odontologia, Departamento de Ciências Básicas, Araçatuba, São Paulo, Brasil.
Univ. Estadual Paulista, Faculdade de Odontologia, Departamento de Cirurgia e Clínica Integrada, Araçatuba, São Paulo, Brasil.
J Appl Oral Sci. 2018 Jul 10;26:e20170470. doi: 10.1590/1678-7757-2017-0470.
The hypothesis of this study was that the peri-implant bone healing of the group of pinealectomized rats would differ from the control group. The samples were subjected to immunohistochemical, microtomographic (total porosity and connectivity density), and fluorochrome (mineralized surface) analyses.
The goal of this study was to investigate the cellular changes and bone remodeling dynamics along the bone/implant interface in pinealectomized rats.
The total of 18 adult male rats (Rattus norvegicus albinus, Wistar) was divided into three groups (n=6): control (CO), pinealectomized without melatonin (PNX) and pinealectomized with melatonin (PNXm). All animals were submitted to the first surgery (pinealectomy), except the CO group. Thirty days after the pinealectomy without melatonin, the second surgery was conducted, in which all animals received an implant in each tibia (36 titanium implants with surface treatment were installed - Implalife® São Paulo, SP, Brazil). By gavage, the rats of the PNX group received the vehicle solution, and the procedure.
Immunohistochemical analysis for runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), osteopontin (OP) and osteocalcin (OC) showed that the bone repair process in the PNXm group was similar to that of the CO group, whereas the PNX group showed a delay. The microtomographic parameters of total porosity [Po(tot)] and bone surface (BS) showed no statistically significant differences, whereas for the connective density (Conn.Dn) a statistical difference was found between the CO and PNXm groups. Fluorochrome analysis of the active mineralized surface showed statistically significant difference between the CO and PNX and between the CO and PNXm groups.
The absence of the pineal gland impaired the bone repair process during osseointegration, however the daily melatonin replacement was able to restore this response.
本研究的假设是，松果体切除大鼠的种植体周围骨愈合情况与对照组不同。样本进行了免疫组织化学、微断层扫描（总孔隙率和连通密度）和荧光标记（矿化表面）分析。
本研究旨在探讨松果体切除大鼠骨/种植体界面的细胞变化和骨重塑动力学。
总共 18 只成年雄性大鼠（白化 Wistar 大鼠）分为三组（n=6）：对照组（CO）、松果体切除但不给予褪黑素（PNX）和松果体切除并给予褪黑素（PNXm）。除 CO 组外，所有动物均进行第一次手术（松果体切除术）。在未给予褪黑素 30 天后，进行第二次手术，所有动物的每只胫骨都植入一个种植体（植入 36 个表面处理的钛种植体-Implalife® São Paulo，SP，巴西）。PNX 组的大鼠通过灌胃给予载体溶液和程序。
RUNX2、碱性磷酸酶（ALP）、骨桥蛋白（OP）和骨钙素（OC）的免疫组织化学分析表明，PNXm 组的骨修复过程与 CO 组相似，而 PNX 组则出现延迟。总孔隙率[Po(tot)]和骨表面（BS）的微断层扫描参数没有统计学差异，而连接密度（Conn.Dn）在 CO 和 PNXm 组之间存在统计学差异。活性矿化表面的荧光标记分析显示，CO 组与 PNX 组和 CO 组与 PNXm 组之间存在统计学差异。
松果体缺失会损害骨整合过程中的骨修复过程，但每天给予褪黑素可以恢复这种反应。
