Monteiro Naara Gabriela, de Oliveira-Filho Odir Nunes, Gandolfo Maria Isabela Lopes, Ervolino da Silva Ana Cláudia, Pitol-Palin Letícia, Botacin Paulo Roberto, Mulinari-Santos Gabriel, de Souza Batista Fábio Roberto, Okamoto Roberta
Department of Diagnosis and Surgery, Araçatuba Dental School, São Paulo State University Júlio de Mesquita Filho, Araçatuba 16015-050, Brazil.
Department of Basic Sciences, Araçatuba Dental School, São Paulo State University Júlio de Mesquita Filho, Araçatuba 16066-840, Brazil.
Biology (Basel). 2025 Jan 29;14(2):139. doi: 10.3390/biology14020139.
Estrogen deficiency contributes to osteoporosis and can therefore compromise the peri-implant bone. Hence, this study evaluated peri-implant bone healing when (RC) was administered orally in ovariectomized and healthy rats. Forty 4-month-old female rats were divided into four groups: SHAM (healthy rats), SHAM/RC (healthy rats treated with RC), OVX (ovariectomized rats), and OVX/RC (ovariectomized rats treated with RC). The oral administration of RC started thirty days after ovariectomy, and implant placement into the rat tibia occurred ninety days after the ovariectomy. Euthanasia occurred sixty days after implantation. The analyses performed included removal torque, RT-PCR, confocal microscopy, and immunolabeling. A significance level of < 0.05 was considered for all tests. The highest reverse torque values were observed in the SHAM/RC group, followed by the OVX/RC group. Confocal microscopy showed the greatest bone biomineralization in the SHAM/RC group, followed by the OVX/RC group. RT-PCR data indicated that RC decreased the RANKL/OPG ratio in both conditions. Immunohistochemistry demonstrated a balance between bone formation and resorption in all groups, especially stimulating osteoblastogenesis in both treated groups. In conclusion, RC enhanced peri-implant bone healing and biomineralization in both healthy and ovariectomized rats, with stronger effects in healthy rats, suggesting that estrogen may enhance its efficacy. These findings support RC's potential as a prophylactic and therapeutic agent.
雌激素缺乏会导致骨质疏松，因此可能会影响种植体周围骨组织。因此，本研究评估了在去卵巢大鼠和健康大鼠口服雷卡索醇（RC）时种植体周围骨组织的愈合情况。40只4月龄雌性大鼠被分为四组：假手术组（健康大鼠）、假手术/RC组（接受RC治疗的健康大鼠）、去卵巢组（去卵巢大鼠）和去卵巢/RC组（接受RC治疗的去卵巢大鼠）。在去卵巢30天后开始口服RC，在去卵巢90天后将种植体植入大鼠胫骨。在植入60天后实施安乐死。所进行的分析包括去除扭矩、逆转录聚合酶链反应（RT-PCR）、共聚焦显微镜检查和免疫标记。所有测试的显著性水平设定为P<0.05。在假手术/RC组中观察到最高的反向扭矩值，其次是去卵巢/RC组。共聚焦显微镜检查显示，假手术/RC组的骨生物矿化程度最高，其次是去卵巢/RC组。RT-PCR数据表明，在两种情况下RC均降低了核因子κB受体活化因子配体（RANKL）与骨保护素（OPG）的比值。免疫组织化学显示所有组的骨形成和骨吸收之间保持平衡，尤其是在两个治疗组中均刺激了成骨细胞生成。总之，RC增强了健康大鼠和去卵巢大鼠种植体周围的骨愈合和生物矿化，在健康大鼠中的作用更强，这表明雌激素可能会增强其疗效。这些发现支持了RC作为预防和治疗药物的潜力。
