a Laboratory of Gene Expression Regulation in Development , Institute of Gene Biology, Russian Academy of Sciences , Moscow , Russia.
d Center of Common Use "Human Proteome" , V.I. Orekhovich Research Institute of Biomedical Chemistry , Moscow , Russia.
Cell Cycle. 2018;17(14):1708-1720. doi: 10.1080/15384101.2018.1496738. Epub 2018 Aug 1.
Paip2 (Poly(A)-binding protein - interacting protein 2) is a conserved metazoan-specific protein that has been implicated in regulating the translation and stability of mRNAs. However, we have found that Paip2 is not restricted to the cytoplasm but is also found in the nucleus in Drosophila embryos, salivary glands, testes, and tissue culture cells. Nuclear Paip2 is associated with chromatin, and in chromatin immunoprecipitation experiments it maps to the promoter regions of active genes. However, this chromatin association is indirect, as it is RNA-dependent. Thus, Paip2 is one more item in the growing list of translation factors that are recruited to mRNAs co-transcriptionally.
Paip2（多聚（A）结合蛋白相互作用蛋白 2）是一种保守的后生动物特异性蛋白，已被证实参与调节 mRNA 的翻译和稳定性。然而，我们发现 Paip2 不仅局限于细胞质，而且还存在于果蝇胚胎、唾液腺、睾丸和组织培养细胞的细胞核中。核内 Paip2 与染色质相关，在染色质免疫沉淀实验中，它定位在活性基因的启动子区域。然而，这种染色质的结合是间接的，因为它依赖于 RNA。因此，Paip2 是越来越多的被招募到共转录 mRNA 中的翻译因子中的一个。
