Friedlander M, Blobel G
Nature. 1985;318(6044):338-43. doi: 10.1038/318338a0.
By deletion of selected segments from a bovine opsin complementary DNA clone and subsequent analysis of transcripts in a cell-free translation-translocation system, we have localized two out of four theoretically conceivable signal sequences required for the integration of opsin into microsomal membranes.