Su Y W, Li F, Zhao Y, Li Y H, Feng Y C, Liu Y M
Guangzhou Prevention and Treatment Center for Occupational disease, Guangzhou 510620, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2018 May 20;36(5):327-330. doi: 10.3760/cma.j.issn.1001-9391.2018.05.002.
To study the effect of sodium aescinate on the development process of lung injury induced by paraquat. Forty-five health adult male SD rats were randomly divided into control group, PQ group, sodium aescinate group, and 15 rats in each group. The PQ group and sodium aescinate group were given a one-time intraperitoneal injection of 18mg/kg body weight of rats PQ, the control group was given the same amout normal saline. Rats in sodium aescinate group were injected 2 mg/kg body weight sodium aescinate into abdominal cavity for 7 days continually, but the same volume of saline was injected into the other groups. Finally, at 7, 14 and 28 days after PQ poisoning, five rats were kills for measuring lung tissue pathological changes and the value of TGF-β(1), TNF-α, hydroxyproline in each group. The expression of TNF-α in serum of 7th day [ (17.03±0.82) ng/ml] and 14th day [ (15.74±0.91) ng/ml] of sodium aescinate group were lower than the corresponding period of PQ groups', and the difference had statistical significance (<0.05) . The expression of TGF-β(1) in serum of 7th day[ (225.93±8.33) ng/ml], 14th day [ (216.62±9.48) ng/ml] and 28th[ (181.41±6.10) ng/ml] of sodium aescinate group were lower than the corresponding period of PQ groups', and the difference had statistical significance (<0.05) . Lung tissue pathological changes showed, compared with control group, inflammatory injury at 7th day and fibrosis degree at 28th of rats' lung reduced on sodium aescinate group. The expression of hydroxyproline in rats' lung of 7th day[ (1.246±0.018) μg/mg], 14th day [ (1.269±0.034) μg/mg] and 28th[ (1.283±0.028) μg/mg] of sodium aescinate group were lower than the corresponding period of PQ groups', and the difference had statistical significance (<0.05) . sodium aescinate could reduce the pulmonary inflammatory injury and hydroxyproline value of PQ poisoning rats, so sodium aescinate could ameliorate lung injury induced by PQ.
研究七叶皂苷钠对百草枯诱导的肺损伤发展过程的影响。将45只健康成年雄性SD大鼠随机分为对照组、百草枯组、七叶皂苷钠组，每组15只。百草枯组和七叶皂苷钠组一次性腹腔注射18mg/kg体重的大鼠百草枯，对照组注射等量生理盐水。七叶皂苷钠组大鼠连续7天腹腔注射2mg/kg体重的七叶皂苷钠，其他组注射等量生理盐水。最后，在百草枯中毒后7天、14天和28天，每组处死5只大鼠，检测肺组织病理变化及各组转化生长因子-β(1)、肿瘤坏死因子-α、羟脯氨酸的值。七叶皂苷钠组第7天[(17.03±0.82)ng/ml]和第14天[(15.74±0.91)ng/ml]血清中肿瘤坏死因子-α的表达低于百草枯组相应时期，差异有统计学意义(<0.05)。七叶皂苷钠组第7天[(225.93±8.33)ng/ml]、第14天[(216.62±9.48)ng/ml]和第28天[(181.41±6.10)ng/ml]血清中转化生长因子-β(1)的表达低于百草枯组相应时期，差异有统计学意义(<0.05)。肺组织病理变化显示，与对照组相比，七叶皂苷钠组大鼠肺在第7天的炎性损伤和第28天的纤维化程度减轻。七叶皂苷钠组大鼠肺第7天[(1.246±0.018)μg/mg]、第14天[(1.269±0.034)μg/mg]和第28天[(1.283±0.028)μg/mg]羟脯氨酸的表达低于百草枯组相应时期，差异有统计学意义(<0.05)。七叶皂苷钠可减轻百草枯中毒大鼠的肺部炎性损伤和羟脯氨酸值，因此七叶皂苷钠可改善百草枯诱导的肺损伤。
