Huang Min, Zhang Ping, Chang Xiu-li, Gu Xi-an, Wu Qing, Zhou Zhi-jun
School of Public Health, Fudan University/National Key Laboratory for Public Health and Safety of MOE, Shanghai 200032, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2009 Aug;27(8):463-7.
To observe the changes of cytokine and NF-kappaB activity in acute paraquat poisoned rats and the effect of PDTC and the mechanism of lung injury caused by paraquat poisoning.
Sprague-Dawley (SD) rats were randomly divided into three groups: Control group (6 rats), PQ group (56 rats) and PQ + PDTC group (46 rats). On the 1st, the 3rd, the 7th, the 14th, the 28th and the 56th day after treatment, the level of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), transforming growth factor-beta 1 (TGF-beta1) and platelet-derived growth factor (PDGF) in serum were detected; the activity of NF-kappaB in lung tissues was detected.
The level of IL-1 beta increased significantly on the 1st, the 3rd, the 7th day in PQ group compared with control group (P<0.01). The content of TGF-beta1 and TNF-alpha in PQ group significantly increased compared with control group (P<0.05 or P<0.01). The level of PDGF significantly increased on the 7th, the 14th, the 28th and the 56th day compared with control group (P<0.01). Meanwhile, IL-1 beta and TNF-alpha were positively correlated with lung coefficient (r=0.37, 0.46, P<0.05 or P<0.01 ); TGF-beta1 and PDGF had positive correlation with hydroxyproline (r=0.56, 0.89, P<0.01). The activity of NF-kappaB in lung tissue of PQ group significantly increased on the 1st, the 3rd, the 7th and 14th day compared with control (P<0.05 or P<0.01). There was a significant decrease of IL-1 beta, TGF-beta1, TNF-alpha and PDGF in PQ + PDTC group compared with PQ group (P<0.05 or P<0.01) in the corresponding sacrifice dates. There was a significant decrease in NF-kappaB activity on the 1st, the 3rd, the 7th day in PQ + PDTC group compared with PQ group (P<0.01).
The cytokine and NF-kappaB could play an important role in lung injury of poisoned rats. PDTC may inhibit the expression of NF-kappaB and further reduce the production of cytokines, alleviate lung injury in acute paraquat poisoned rats.
观察急性百草枯中毒大鼠细胞因子及核因子-κB（NF-κB）活性变化，以及吡咯烷二硫代氨基甲酸盐（PDTC）的作用和百草枯中毒致肺损伤的机制。
将Sprague-Dawley（SD）大鼠随机分为三组：对照组（6只大鼠）、百草枯组（56只大鼠）和百草枯+PDTC组（46只大鼠）。于治疗后第1、3、7、14、28和56天，检测血清中白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、转化生长因子-β1（TGF-β1）和血小板衍生生长因子（PDGF）水平；检测肺组织中NF-κB活性。
与对照组相比，百草枯组第1、3、7天IL-1β水平显著升高（P<0.01）。百草枯组TGF-β1和TNF-α含量与对照组相比显著增加（P<0.05或P<0.01）。与对照组相比，第7、14、28和56天PDGF水平显著升高（P<0.01）。同时，IL-1β和TNF-α与肺系数呈正相关（r=0.37，0.46，P<0.05或P<0.01）；TGF-β1和PDGF与羟脯氨酸呈正相关（r=0.56，0.89，P<0.01）。与对照组相比，百草枯组肺组织中NF-κB活性在第1、3、7和14天显著升高（P<0.05或P<0.01）。在相应处死日期，百草枯+PDTC组与百草枯组相比，IL-1β、TGF-β1、TNF-α和PDGF显著降低（P<0.05或P<0.01）。与百草枯组相比，百草枯+PDTC组第1、3、7天NF-κB活性显著降低（P<0.01）。
细胞因子和NF-κB在中毒大鼠肺损伤中可能起重要作用。PDTC可能抑制NF-κB表达并进一步减少细胞因子产生，减轻急性百草枯中毒大鼠的肺损伤。
