Liu Feng, Cao Yue-Ping
Plant Science Department, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, P.R. China.
J Microbiol Biotechnol. 2018 Aug 28;28(8):1384-1390. doi: 10.4014/jmb.1801.01026.
Glyphosate inhibits the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in the shikimate pathway. A mutant of EPSPS from sp. was identified using site-directed mutagenesis (SDM). The mutant significantly improved glyphosate resistance. The mutant had mutations in three amino acids: Gly97 to Ala, Thr 98 to Ile and Pro 102 to Ser. These mutation sites in have been studied as significant active sites of glyphosate resistance. However, in our research they were found to jointly contribute to the improvement of glyphosate tolerance. In addition, the level of glyphosate tolerance in transgenic confirmed the potentiality of the mutant in breeding glyphosate-resistant plants.
草甘膦抑制莽草酸途径中的靶标酶5-烯醇丙酮酰莽草酸-3-磷酸合酶（EPSPS）。使用定点诱变（SDM）从[具体物种]中鉴定出EPSPS的一个突变体。该突变体显著提高了对草甘膦的抗性。该突变体在三个氨基酸上发生了突变：甘氨酸97突变为丙氨酸，苏氨酸98突变为异亮氨酸，脯氨酸102突变为丝氨酸。[具体物种]中的这些突变位点已被研究为草甘膦抗性的重要活性位点。然而，在我们的研究中发现它们共同有助于提高对草甘膦的耐受性。此外，转基因[具体物种]中的草甘膦耐受水平证实了该突变体在培育抗草甘膦植物方面的潜力。
