Chen Yu-Fan, Song Bing, Liao Zhen-Ting, Deng Zhong-Hao, Wu De-Sheng, Feng Shu-Hao, Zhao Liang
Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2018 Jun 20;38(6):716-722. doi: 10.3969/j.issn.1673-4254.2018.06.12.
To evaluate the effect of a novel biomaterial in repairing large cranial defects in rats.
Eighteen SD rats were used to establish rat modes of large cranial defect (8 mm in diameter). The rat models were randomized into 3 groups and the cranial defects were repaired using different scaffold materials, namely CPC paste prepared with distilled water (CPC control group), CPC paste mixed with 10% chitosan (CPC/CN group), or CPC paste with 10% chitosan and 300 mg adenosine (CPC/CN/AD group). The defects were examined 12 weeks after the surgery with X-ray, CT, HE staining and quantitative assessments.
X-ray showed that the defect was repaired in all the groups. The fracture line became obscure and the defects were almost fully repaired by regenerated bone tissues in CPC/CN/AD group, which was consistent with CT findings. In all the 3 groups, HE staining revealed the presence of new bones in the defects and new vessels in and around the new bones without inflammatory cells. The new bone area was significantly greater in CPC/CN/AD group than in CPC/CN group and CPC control group (P<0.05). The new vessel density was the highest in CPC/CN/AD group (P>0.05) but similar between CPC/CN group and CPC control group (P>0.05).
This novel calcium phosphate cement pre-loaded with chitosan and small molecule adenosine can better promote bone regeneration than calcium phosphate cement for repairing large bone defects to serve as a good replacement material for bone regeneration.
评估一种新型生物材料修复大鼠大型颅骨缺损的效果。
选用18只SD大鼠建立直径8毫米的大型颅骨缺损大鼠模型。将大鼠模型随机分为3组，使用不同的支架材料修复颅骨缺损，即用蒸馏水制备的磷酸钙骨水泥（CPC对照组）、与10%壳聚糖混合的磷酸钙骨水泥（CPC/CN组）或含10%壳聚糖和300毫克腺苷的磷酸钙骨水泥（CPC/CN/AD组）。术后12周通过X射线、CT、苏木精-伊红染色（HE染色）及定量评估对缺损情况进行检查。
X射线显示所有组的缺损均得到修复。在CPC/CN/AD组中骨折线变模糊，缺损几乎完全被再生骨组织修复，这与CT结果一致。在所有3组中，HE染色显示缺损处有新骨形成，新骨内及周围有新血管，且无炎性细胞。CPC/CN/AD组的新骨面积显著大于CPC/CN组和CPC对照组（P<0.05）。CPC/CN/AD组的新血管密度最高（P>0.05），但CPC/CN组和CPC对照组之间相似（P>0.05）。
这种预先负载壳聚糖和小分子腺苷的新型磷酸钙骨水泥在修复大型骨缺损方面比磷酸钙骨水泥能更好地促进骨再生，可作为骨再生的良好替代材料。
