Li Juan, Li Hang, Lv Xiaoting, Yang Zitai, Gao Min, Bi Yanhong, Zhang Ziwei, Wang Shengli, Cui Zhigang, Zhou Baosen, Yin Zhihua
1Department of Epidemiology, School of Public Health, China Medical University, Shenyang, 110122 People's Republic of China.
2Key Laboratory of Cancer Etiology and Intervention, University of Liaoning Province, No. 77 Puhe Road, Shenyang North New Area, Shenyang, 110122 People's Republic of China.
Cancer Cell Int. 2018 Jul 4;18:91. doi: 10.1186/s12935-018-0591-2. eCollection 2018.
Long non-coding RNAs play pivotal roles in the carcinogenesis of multiple types of cancers. This study is firstly to evaluate influence of rs4848320 and rs1110839 polymorphisms in long non-coding RNA AC016683.6 on the susceptibility of lung cancer.
The present study was a hospital-based case-control study with 434 lung cancer patients and 593 cancer-free controls. Genotyping of the two SNPs detected by Taqman allelic discrimination method.
There were no statistically significant associations between rs4848320 and rs1110839 polymorphisms in AC016683.6 and risk of lung cancer in overall population. However, in the smoking population, rs4848320 and rs1110839 polymorphisms significantly increased the risk of lung cancer in dominant and homozygous models (Rs4848320: P = 0.029; Rs1110839: P = 0.034), respectively. In male population, rs1110839 genetic variant was related to the risk of lung cancer in all genetic models (GG vs. TT: P = 0.008; Dominant model: P = 0.029; Recessive model: P = 0.027) rather than heterozygous model. The crossover analyses provided rs4848320 and rs1110839 risk genotypes carriers combined with smoking exposure 2.218-fold, 1.755-fold increased risk of lung cancer (Rs4848320: P = 0.005; Rs1110839: P = 0.017). Additionally, there were significantly positive multiplicative interaction of rs4848320 polymorphism with smoking status, with adjusted OR of 2.244 (1.162-4.334), but rs1110839 polymorphism did not exist.
Rs4848320 and rs1110839 polymorphisms may be associated with lung cancer susceptibility. Interaction of rs4848320 risk genotypes with smoking exposure may strengthen the risk effect on lung cancer.
长链非编码RNA在多种癌症的发生发展中起关键作用。本研究首次评估长链非编码RNA AC016683.6中rs4848320和rs1110839多态性对肺癌易感性的影响。
本研究是一项基于医院的病例对照研究，纳入434例肺癌患者和593例无癌对照。采用Taqman等位基因分型法对两个单核苷酸多态性进行基因分型。
AC016683.6中rs4848320和rs1110839多态性与总体人群肺癌风险之间无统计学显著关联。然而，在吸烟人群中，rs4848320和rs1110839多态性在显性和纯合模型中分别显著增加肺癌风险（rs4848320：P = 0.029；rs1110839：P = 0.034）。在男性人群中，rs1110839基因变异在所有遗传模型中均与肺癌风险相关（GG与TT：P = 0.008；显性模型：P = 0.029；隐性模型：P = 0.027），而非杂合模型。交叉分析显示，rs4848320和rs1110839风险基因型携带者与吸烟暴露相结合，患肺癌风险分别增加2.218倍、1.755倍（rs4848320：P = 0.005；rs1110839：P = 0.017）。此外，rs4848320多态性与吸烟状态存在显著的正相乘交互作用，调整后的比值比为2.244（1.162 - 4.334），但rs1110839多态性不存在这种情况。
rs4848320和rs1110839多态性可能与肺癌易感性相关。rs4848320风险基因型与吸烟暴露的相互作用可能会增强对肺癌的风险影响。
