• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

陆地棉（Gossypium hirsutum L.）中一种微管相关蛋白GhCLASP2的鉴定与功能表征

Identification and Functional Characterization of a Microtubule-Associated Protein, GhCLASP2, From Upland Cotton ( L.).

作者信息

Zhu Shou-Hong, Xue Fei, Li Yan-Jun, Liu Feng, Zhang Xin-Yu, Zhao Lan-Jie, Sun Yu-Qiang, Zhu Qian-Hao, Sun Jie

机构信息

The Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi, China.

State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, China.

出版信息

Front Plant Sci. 2018 Jun 27;9:882. doi: 10.3389/fpls.2018.00882. eCollection 2018.

DOI:10.3389/fpls.2018.00882
PMID:29997641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6030384/
Abstract

Cytoplasmic linker-associated proteins (CLASPs) are microtubule-associated proteins (MAPs) involved in regulation of dynamics of microtubules (MTs) that play an important role in plant growth and development. In this study, we identified cotton genes and investigated the function of . was mainly expressed in stem and developing fibers, especially in fibers of the secondary cell wall deposition stage. Ectopic expression of in increased the branching number of leaf trichomes and rescued the defective phenotypes of . In cotton, overexpression of increased fiber strength, probably related to enhanced expression levels of tubulin, cellulose synthase, and expansin genes. Suppression of caused shorter internodes and semi-dwarfism, abnormal flower stigma, aborted anthers without pollen grains, and sterility. These changed phenotypes were similar to those observed in the mutant. GhCLASP2 was co-localized with MTs according to transient experiment. These results suggest that functions similarly as , acting as a MAP and controlling cotton growth and development by regulating MTs.

摘要

细胞质连接相关蛋白（CLASPs）是微管相关蛋白（MAPs），参与微管（MTs）动力学调控，而微管在植物生长发育中起重要作用。在本研究中，我们鉴定了棉花基因并研究了其功能。主要在茎和发育中的纤维中表达，尤其是在次生细胞壁沉积阶段的纤维中。在中异位表达增加了叶毛状体的分支数并挽救了的缺陷表型。在棉花中，过表达增加了纤维强度，这可能与微管蛋白、纤维素合酶和扩张蛋白基因表达水平的提高有关。抑制导致节间缩短和半矮化、花柱头异常、花药无花粉粒且败育以及不育。这些变化的表型与在突变体中观察到的相似。根据瞬时实验，GhCLASP2与微管共定位。这些结果表明，其功能与相似，作为一种MAP并通过调节微管来控制棉花的生长发育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/781cca40e1fe/fpls-09-00882-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/dd3eb1f8e8bf/fpls-09-00882-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/6c3b4429cb2d/fpls-09-00882-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/7bf9f3eb244d/fpls-09-00882-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/fe37c765c704/fpls-09-00882-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/aff61024a949/fpls-09-00882-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/02adff3d5569/fpls-09-00882-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/781cca40e1fe/fpls-09-00882-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/dd3eb1f8e8bf/fpls-09-00882-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/6c3b4429cb2d/fpls-09-00882-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/7bf9f3eb244d/fpls-09-00882-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/fe37c765c704/fpls-09-00882-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/aff61024a949/fpls-09-00882-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/02adff3d5569/fpls-09-00882-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/47ef/6030384/781cca40e1fe/fpls-09-00882-g007.jpg

相似文献

1
Identification and Functional Characterization of a Microtubule-Associated Protein, GhCLASP2, From Upland Cotton ( L.).陆地棉（Gossypium hirsutum L.）中一种微管相关蛋白GhCLASP2的鉴定与功能表征
Front Plant Sci. 2018 Jun 27;9:882. doi: 10.3389/fpls.2018.00882. eCollection 2018.
2
Genome-wide identification and characterization of the CLASP_N gene family in upland cotton ( L.).在陆地棉（L.）中全基因组鉴定和特征分析 CLASP_N 基因家族。
PeerJ. 2022 Jan 3;10:e12733. doi: 10.7717/peerj.12733. eCollection 2022.
3
Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber.棉花（陆地棉）纤维中驱动蛋白基因GhKIS13A1的表达及功能分析
BMC Biotechnol. 2017 Jun 12;17(1):50. doi: 10.1186/s12896-017-0373-2.
4
Gene expression profile analysis of Ligon lintless-1 (Li1) mutant reveals important genes and pathways in cotton leaf and fiber development.Ligon lintless-1 (Li1) 突变体的基因表达谱分析揭示了棉花叶片和纤维发育中的重要基因和途径。
Gene. 2014 Feb 10;535(2):273-85. doi: 10.1016/j.gene.2013.11.017. Epub 2013 Nov 23.
5
The cotton protein GhIQD21 interacts with GhCaM7 and modulates organ morphogenesis in Arabidopsis by influencing microtubule stability.棉花蛋白 GhIQD21 与 GhCaM7 相互作用，并通过影响微管稳定性来调节拟南芥的器官形态发生。
Plant Cell Rep. 2023 Jun;42(6):1025-1038. doi: 10.1007/s00299-023-03010-z. Epub 2023 Apr 3.
6
Functional characterization of a basic helix-loop-helix (bHLH) transcription factor GhDEL65 from cotton (Gossypium hirsutum).棉花（陆地棉）中一种碱性螺旋-环-螺旋（bHLH）转录因子GhDEL65的功能特性分析
Physiol Plant. 2016 Oct;158(2):200-12. doi: 10.1111/ppl.12450. Epub 2016 Jun 6.
7
Identification of Gene Family in Upland Cotton and Its Functional Analysis in Cotton Fiber Development.鉴定陆地棉基因家族及其在棉花纤维发育中的功能分析。
Genes (Basel). 2019 Jul 4;10(7):508. doi: 10.3390/genes10070508.
8
Genome-wide identification and characterization of TALE superfamily genes in cotton reveals their functions in regulating secondary cell wall biosynthesis.棉花全基因组中 TALE 超家族基因的鉴定和特征分析揭示了它们在调控次生细胞壁生物合成中的功能。
BMC Plant Biol. 2019 Oct 17;19(1):432. doi: 10.1186/s12870-019-2026-1.
9
Comparative transcriptome analysis of cotton fiber development of Upland cotton (Gossypium hirsutum) and Chromosome Segment Substitution Lines from G. hirsutum × G. barbadense.陆地棉（Gossypium hirsutum）与陆地棉×海岛棉染色体片段代换系棉纤维发育的比较转录组分析
BMC Genomics. 2017 Sep 8;18(1):705. doi: 10.1186/s12864-017-4077-8.
10
Transcriptome Analysis Suggests That Chromosome Introgression Fragments from Sea Island Cotton () Increase Fiber Strength in Upland Cotton ().转录组分析表明，海岛棉的染色体渐渗片段提高了陆地棉的纤维强度。
G3 (Bethesda). 2017 Oct 5;7(10):3469-3479. doi: 10.1534/g3.117.300108.

引用本文的文献

1
An explanation of the mystifying bakanae disease narrative for tomorrow's rice.为明日之稻米解读神秘的恶苗病故事。
Front Microbiol. 2023 Apr 18;14:1153437. doi: 10.3389/fmicb.2023.1153437. eCollection 2023.
2
The cotton protein GhIQD21 interacts with GhCaM7 and modulates organ morphogenesis in Arabidopsis by influencing microtubule stability.棉花蛋白 GhIQD21 与 GhCaM7 相互作用，并通过影响微管稳定性来调节拟南芥的器官形态发生。
Plant Cell Rep. 2023 Jun;42(6):1025-1038. doi: 10.1007/s00299-023-03010-z. Epub 2023 Apr 3.
3
Transcriptomic analysis of bakanae disease resistant and susceptible rice genotypes in response to infection by Fusarium fujikuroi.

本文引用的文献

1
Cell polarity in plants: the Yin and Yang of cellular functions.植物中的细胞极性：细胞功能的阴阳之道。
Curr Opin Plant Biol. 2017 Feb;35:105-110. doi: 10.1016/j.pbi.2016.11.015. Epub 2016 Dec 3.
2
Localized Mechanical Stress Promotes Microtubule Rescue.局部机械应力促进微管救援。
Curr Biol. 2016 Dec 19;26(24):3399-3406. doi: 10.1016/j.cub.2016.10.048. Epub 2016 Dec 1.
3
Differential Gene Expression and Protein Phosphorylation as Factors Regulating the State of the Arabidopsis SNX1 Protein Complexes in Response to Environmental Stimuli.
对感染藤仓镰孢菌的抗恶苗病和感恶苗病水稻基因型进行转录组分析。
Mol Biol Rep. 2022 Dec;49(12):11959-11972. doi: 10.1007/s11033-022-07877-1. Epub 2022 Oct 21.
4
Comparative transcriptome analysis reveals key pathways and genes involved in trichome development in tea plant ().比较转录组分析揭示了茶树毛状体发育过程中的关键途径和基因。
Front Plant Sci. 2022 Sep 23;13:997778. doi: 10.3389/fpls.2022.997778. eCollection 2022.
5
Microtubules play a crucial role in regulating actin organization and cell initiation in cotton fibers.微管在调控棉花纤维中的肌动蛋白组织和细胞起始方面发挥着关键作用。
Plant Cell Rep. 2022 Apr;41(4):1059-1073. doi: 10.1007/s00299-022-02837-2. Epub 2022 Feb 26.
6
Genome-wide identification and characterization of the CLASP_N gene family in upland cotton ( L.).在陆地棉（L.）中全基因组鉴定和特征分析 CLASP_N 基因家族。
PeerJ. 2022 Jan 3;10:e12733. doi: 10.7717/peerj.12733. eCollection 2022.
7
Cell biology of primary cell wall synthesis in plants.植物初生细胞壁合成的细胞生物学。
Plant Cell. 2022 Jan 20;34(1):103-128. doi: 10.1093/plcell/koab249.
8
Association Mapping of Verticillium Wilt Disease in a Worldwide Collection of Cotton ( L.).全球棉花（陆地棉）黄萎病的关联作图
Plants (Basel). 2021 Feb 5;10(2):306. doi: 10.3390/plants10020306.
9
Comprehensive Genome-Wide Analysis of Thaumatin-Like Gene Family in Four Cotton Species and Functional Identification of Involved in Regulating Tolerance to and Drought.四种棉花中类甜蛋白基因家族的全基因组综合分析及参与调控对盐和干旱耐受性的功能鉴定
Front Plant Sci. 2020 Oct 20;11:575015. doi: 10.3389/fpls.2020.575015. eCollection 2020.
差异基因表达和蛋白质磷酸化作为调节拟南芥SNX1蛋白复合体状态以响应环境刺激的因素。
Front Plant Sci. 2016 Sep 26;7:1456. doi: 10.3389/fpls.2016.01456. eCollection 2016.
4
Sequencing of allotetraploid cotton (Gossypium hirsutum L. acc. TM-1) provides a resource for fiber improvement.四倍体棉花（陆地棉 TM-1）基因组测序为纤维改良提供资源。
Nat Biotechnol. 2015 May;33(5):531-7. doi: 10.1038/nbt.3207. Epub 2015 Apr 20.
5
Genome sequence of cultivated Upland cotton (Gossypium hirsutum TM-1) provides insights into genome evolution.栽培棉（陆地棉 TM-1）基因组序列揭示了基因组进化的奥秘。
Nat Biotechnol. 2015 May;33(5):524-30. doi: 10.1038/nbt.3208. Epub 2015 Apr 20.
6
Kinesin-4 Functions in Vesicular Transport on Cortical Microtubules and Regulates Cell Wall Mechanics during Cell Elongation in Plants.驱动蛋白-4 在皮层微管上的囊泡运输中的功能及其在植物细胞伸长过程中对细胞壁力学的调控作用。
Mol Plant. 2015 Jul;8(7):1011-23. doi: 10.1016/j.molp.2015.01.004. Epub 2015 Jan 17.
7
CLASP: a microtubule-based integrator of the hormone-mediated transitions from cell division to elongation.CLASP：一种基于微管的整合因子，参与激素介导的从细胞分裂到伸长的转变过程。
Curr Opin Plant Biol. 2014 Dec;22:149-158. doi: 10.1016/j.pbi.2014.11.003.
8
Control of oriented cell division in the Arabidopsis embryo.拟南芥胚胎中定向细胞分裂的控制。
Curr Opin Plant Biol. 2015 Feb;23:25-30. doi: 10.1016/j.pbi.2014.10.004. Epub 2014 Oct 25.
9
Microtubule organization and microtubule-associated proteins in plant cells.植物细胞中的微管组织和微管相关蛋白。
Int Rev Cell Mol Biol. 2014;312:1-52. doi: 10.1016/B978-0-12-800178-3.00001-4.
10
Genome sequence of the cultivated cotton Gossypium arboreum.栽培棉种陆地棉基因组序列。
Nat Genet. 2014 Jun;46(6):567-72. doi: 10.1038/ng.2987. Epub 2014 May 18.