Serviço de Biotecnologia Vegetal, Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil.
Serviço de Fitoquímica e Prospecção Farmacêutica, Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil.
Phytother Res. 2018 Oct;32(10):2021-2033. doi: 10.1002/ptr.6133. Epub 2018 Jul 12.
Species of Aspidosperma are traditionally used to treat malaria, leishmaniasis, microbial, and inflammatory diseases. Aspidosperma subincanum Mart. known as "guatambu" is used in Brazilian traditional medicine to treat diabetes, hypercholesterolemia, and digestive diseases. Its tonic properties have been employed by the indigenous populations to stimulate the circulatory and genitourinary tracts and to improve respiratory function as well as to relieve spasms and to reduce fever. The species is known to contain antitumoural and antimalarial indole alkaloids. In the present study, various less explored biological activities of extracts from leaves and branches of A. subincanum were investigated, that is, inhibition of acetylcholinesterase as well as antioxidant and antibacterial activity. Twenty-one known indole alkaloids from this species were targeted for predicting the inhibition of acetylcholinesterase, and their biological activities were collected from the literature. Through in silico the prediction, the indole alkaloids uleine and derivatives demonstrated a strong probability of being able to inhibit the acetylcholinesterase enzyme, as well as the olivacine derivatives 3,4-dihydroolivacine and N-methyl-tetrahydro-olivacine (guatambuine), and the subincanadines C and E. Indeed, the extracts of A. subincanum showed acetylcholinesterase inhibitory activity, antioxidant activity in the lipid peroxidation assay, and antimicrobial activity against Staphylococcus aureus ATCC 25923, and their pharmacological properties should be explored further.
该属植物传统上被用于治疗疟疾、利什曼病、微生物和炎症性疾病。Aspidosperma subincanum Mart. 俗称“guatambu”，在巴西传统医学中用于治疗糖尿病、高胆固醇血症和消化系统疾病。其滋补特性被土著居民用于刺激循环和生殖泌尿系统，改善呼吸功能，缓解痉挛和降低体温。该物种被认为含有抗肿瘤和抗疟吲哚生物碱。在本研究中，研究了该属植物叶和枝的提取物的各种较少探索的生物活性，即乙酰胆碱酯酶抑制活性以及抗氧化和抗菌活性。针对该物种的 21 种已知吲哚生物碱进行了预测乙酰胆碱酯酶抑制作用的研究，并从文献中收集了它们的生物活性数据。通过计算机预测，乌莱因和衍生物吲哚生物碱具有很强的抑制乙酰胆碱酯酶的可能性，以及奥利瓦辛衍生物 3,4-二氢奥利瓦辛和 N-甲基-四氢-奥利瓦辛（guatambuine）和 subincanadines C 和 E。事实上，A. subincanum 的提取物表现出乙酰胆碱酯酶抑制活性、脂质过氧化测定中的抗氧化活性和对金黄色葡萄球菌 ATCC 25923 的抗菌活性，其药理特性应进一步探索。
