Duh Q Y, Gum E T, Gerend P L, Raper S E, Clark O H
Surgery. 1985 Dec;98(6):1000-7.
Epidermal growth factor (EGF) stimulates DNA synthesis and proliferation of thyroid cells in culture and may have an important role in the regulation of normal and neoplastic thyroid cell growth. We therefore studied paired normal and neoplastic thyroid tissue from eight patients for the presence of EGF receptors using a radioreceptor assay. 125I EGF binds to a particulate membrane fraction from both normal and neoplastic thyroid tissue with high affinity (dissociation constant ranged from 0.5 to 16.7 nmol/L). The binding is saturable, and maximal binding is achieved within 40 minutes at 37 degrees C and pH 7.5. This EGF binding is specific since it is competitively inhibited by unlabeled EGF but not by other hormones (thyrotropin, insulin, glucagon, and transferrin). The binding of EGF to thyroid neoplasms is higher than the binding to normal thyroid tissue (p less than 0.05). Thyroid tumors with a poorer prognosis appear to have higher EGF binding compared with adjacent normal thyroid tissue than have tumors with a better prognosis. EGF may have a role in the regulation of normal and neoplastic thyroid cell growth. Characterization of EGF receptors may help predict the clinical course of patients with malignant thyroid neoplasms.
表皮生长因子（EGF）可刺激培养的甲状腺细胞的DNA合成及增殖，在正常和肿瘤性甲状腺细胞生长的调节中可能发挥重要作用。因此，我们使用放射受体分析法研究了8例患者配对的正常和肿瘤性甲状腺组织中EGF受体的存在情况。125I EGF以高亲和力（解离常数范围为0.5至16.7 nmol/L）与正常和肿瘤性甲状腺组织的微粒膜部分结合。这种结合是可饱和的，在37℃和pH 7.5条件下40分钟内可达到最大结合。这种EGF结合具有特异性，因为它可被未标记的EGF竞争性抑制，但不受其他激素（促甲状腺激素、胰岛素、胰高血糖素和转铁蛋白）抑制。EGF与甲状腺肿瘤的结合高于与正常甲状腺组织的结合（p<0.05）。与预后较好的肿瘤相比，预后较差的甲状腺肿瘤与相邻正常甲状腺组织相比，似乎具有更高的EGF结合。EGF可能在正常和肿瘤性甲状腺细胞生长的调节中发挥作用。EGF受体的特性分析可能有助于预测甲状腺恶性肿瘤患者的临床病程。
