Krasil'nikov I V, Kazantseva V A, Ivanova O E, Savinskaia S S, Balaian M S
Vopr Virusol. 1985 Sep-Oct;30(5):608-10.
The possibility of isolation of different viruses, hepatitis A virus among them, from water by means of adsorption chromatography on porous silica was demonstrated. Adsorption of viruses on the surface of silica depended on the kind of sorbent, water pH, and the rate of water flow through the column. The maximum elution of the adsorbed virus was observed using buffer solution with pH at least 11.0 and containing 1-2M NaCl. These conditions for adsorption-elution were found to be universal for different enterovirus types. In the field, the developed method was used for testing water specimens from various water bodies (sewage, river water, tap water). No viruses were found in 11 native specimens. Eluates of 4 out of 11 specimens were found to contain enteroviruses, hepatitis A virus among them.
已证明通过在多孔硅胶上进行吸附色谱法从水中分离出不同病毒（包括甲型肝炎病毒）的可能性。病毒在硅胶表面的吸附取决于吸附剂的种类、水的pH值以及水流过柱子的速率。使用pH至少为11.0且含有1-2M氯化钠的缓冲溶液可观察到吸附病毒的最大洗脱量。发现这些吸附-洗脱条件对不同类型的肠道病毒具有普遍性。在实际应用中，所开发的方法用于检测来自各种水体（污水、河水、自来水）的水样。11份原始水样中未发现病毒。11份水样中的4份洗脱液被发现含有肠道病毒，其中包括甲型肝炎病毒。
