Sepp Krisztian, Laszlo Anna M, Molnar Zsolt, Serester Andrea, Alapi Tunde, Galfi Marta, Valkusz Zsuzsanna, Radacs Marianna
First Department of Medicine, Faculty of Medicine, University of Szeged, Szeged, Hungary.
Department of Biometrics and Agricultural Informatics, Faculty of Horticultural Science, Szent István University, Budapest, Hungary.
Int J Endocrinol. 2018 May 29;2018:7493418. doi: 10.1155/2018/7493418. eCollection 2018.
Uron herbicides polluting the environment represent a serious concern for environmental health and may be regarded as endocrine-disrupting compounds (EDCs), which influence the regulation of human homeostasis. We aimed to investigate the effect of EDC urons (phenuron: PU, monuron: MU, and diuron: DU) and chlorobenzenes on the basal release of the adrenocorticotropic hormone (ACTH), which is a part of the adenohypophysis-adrenocortical axis. Hormone secretion in the presence of EDC was studied in two cell types: normal adenohypophysis cells (AdH) and cells of prolactinomas (PRLOMA). PRLOMA was induced in female Wistar rats by subcutaneously injecting them with estrone acetate for 6 months. AdH and PRLOMA were separated from treated and untreated experimental animals, dissociated enzymatically and mechanically in order to create monolayer cell cultures, which served as an experimental model. We investigated the effects of ED agents separately and in combination on ACTH and prolactin (PRL) release through the hypophyseal-adrenal axis. Hormone determination was carried out by the luminescent immunoassay and the radioimmunoassay methods. Our results showed that (1) uron agents separately did not change ACTH and PRL release in AdH culture; (2) ACTH secretion in arginine vasopressin- (AVP-) activated AdH cells was significantly increased by EDC treatment; (3) ED agents increased the basal hormone release (ACTH, PRL) in PRLOMA cells; and (4) EDC exposure increased ACTH release in AVP-activated PRLOMA cells. We conclude that the herbicides PU, MU, and DU carry EDC effects and show human toxicity potential.
污染环境的脲类除草剂对环境卫生构成严重威胁，可能被视为内分泌干扰化合物（EDC），会影响人体稳态的调节。我们旨在研究EDC脲类（非草隆：PU、灭草隆：MU和敌草隆：DU）和氯苯对促肾上腺皮质激素（ACTH）基础释放的影响，ACTH是腺垂体-肾上腺皮质轴的一部分。在两种细胞类型中研究了EDC存在时的激素分泌：正常腺垂体细胞（AdH）和催乳素瘤细胞（PRLOMA）。通过给雌性Wistar大鼠皮下注射醋酸雌酮6个月诱导产生PRLOMA。将AdH和PRLOMA从经处理和未经处理的实验动物中分离出来，通过酶解和机械解离以创建单层细胞培养物，作为实验模型。我们分别和联合研究了ED剂对通过垂体-肾上腺轴的ACTH和催乳素（PRL）释放的影响。通过发光免疫测定法和放射免疫测定法进行激素测定。我们的结果表明：（1）脲类试剂单独作用时不会改变AdH培养物中ACTH和PRL的释放；（2）EDC处理可显著增加精氨酸加压素（AVP）激活的AdH细胞中ACTH的分泌；（3）ED剂增加了PRLOMA细胞中基础激素的释放（ACTH、PRL）；（4）EDC暴露增加了AVP激活的PRLOMA细胞中ACTH的释放。我们得出结论，除草剂PU、MU和DU具有EDC效应，并显示出对人体的潜在毒性。
