Wang Shi, Tian Shenglan, Li Mingzhe, Li Zhichao
1Department of Pediatrics, Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430016 Hubei China.
2The Hospital of Wuhan University of Science and Technology, Wuhan, 430065 Hubei China.
3 Biotech. 2018 Jul;8(7):303. doi: 10.1007/s13205-018-1311-2. Epub 2018 Jun 30.
The present study investigated the anti-arthritic effects of methionine in neonatal rats. Rats were divided into four groups, with six rats in each group. The rats were administered methionine (150- or 300-mg/kg body weight) orally for 45 consecutive days. The expression levels of catalase, superoxide dismutase (SOD), reduced glutathione (GSH), lipid peroxidation, glutathione peroxidase (Gpx), prostaglandin E (PGE), matrix metalloproteinase-3, uric acid, nitric oxide (NO), ceruloplasmin, inducible nitric oxide synthase (iNOS), and nuclear factor (NF)-κB were determined in rheumatoid arthritis-induced neonatal rats. The levels of SOD, catalase, Gpx, and GSH were substantially reduced in control rats, while the levels of other parameters were increased in control neonatal rats. However, methionine supplementation significantly increased (more than 40%) the levels of SOD, catalase, Gpx, and GSH in neonatal rats. The levels of lipid peroxidation, uric acid, ceruloplasmin, NO, and PGE were significantly reduced following methionine supplementation. Furthermore, NF-κB mRNA expression was substantially reduced up to 51.7% in the 300-mg/kg methionine group, whereas the mRNA expression of iNOS was reduced up to 43.5% in the 300-mg/kg methionine group. NF-κB protein expression was substantially reduced up to 45.8% in the 300-mg/kg methionine group, whereas the protein expression of iNOS was reduced up to 45.4% in the 300-mg/kg methionine group. Taken together, these data suggest that methionine supplementation was effective against rheumatoid arthritis.
本研究调查了蛋氨酸对新生大鼠的抗关节炎作用。大鼠被分为四组，每组六只。连续45天给大鼠口服蛋氨酸（150或300毫克/千克体重）。测定类风湿性关节炎诱导的新生大鼠中过氧化氢酶、超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、脂质过氧化、谷胱甘肽过氧化物酶（Gpx）、前列腺素E（PGE）、基质金属蛋白酶-3、尿酸、一氧化氮（NO）、铜蓝蛋白、诱导型一氧化氮合酶（iNOS）和核因子（NF）-κB的表达水平。对照组大鼠中SOD、过氧化氢酶、Gpx和GSH的水平显著降低，而其他参数在对照新生大鼠中升高。然而，补充蛋氨酸显著提高了新生大鼠中SOD、过氧化氢酶、Gpx和GSH的水平（超过40%）。补充蛋氨酸后，脂质过氧化、尿酸、铜蓝蛋白、NO和PGE的水平显著降低。此外，在300毫克/千克蛋氨酸组中，NF-κB mRNA表达大幅降低达51.7%，而在300毫克/千克蛋氨酸组中，iNOS的mRNA表达降低达43.5%。在300毫克/千克蛋氨酸组中，NF-κB蛋白表达大幅降低达45.8%，而在300毫克/千克蛋氨酸组中，iNOS的蛋白表达降低达45.4%。综上所述，这些数据表明补充蛋氨酸对类风湿性关节炎有效。
