从茶到功能性食品：探索菜豆属植物抗类风湿关节炎作用及其潜在机制
From Tea to Functional Foods: Exploring Bunge for Anti-Rheumatoid Arthritis and Unraveling Its Potential Mechanisms.
作者信息
Dong Xin, Wang Zhi, Fu Yao, Tian Yuxin, Xue Peifeng, Wang Yuewu, Yang Feiyun, Li Guojing, Wang Ruigang
机构信息
Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia, Inner Mongolia Agricultural University, Hohhot 010018, China.
Department of Pharmacy, Inner Mongolia Medical University, Jinshan Development Zone, Hohhot 010110, China.
出版信息
Nutrients. 2024 Dec 13;16(24):4311. doi: 10.3390/nu16244311.
BACKGROUND
Bunge (CM) shows promising potential for managing rheumatoid arthritis (RA) and digestive disorders, attributed to its rich content of bioactive compounds such as polyphenols and flavonoids. Despite its common use in herbal tea, the specific mechanisms underlying CM's anti-inflammatory and joint-protective effects remain unclear, limiting its development as a functional food. This study investigated the effects of aqueous CM extract on RA in collagen-induced arthritis (CIA) rats and explored the underlying mechanisms.
METHODS
Forty-eight female Sprague-Dawley rats were randomly assigned to six groups ( = 8): normal control, CIA model, methotrexate (MTX), and CM high-, middle-, and low-dose groups. Anti-inflammatory and joint-protective effects were evaluated using biochemical and histological analyses. To elucidate the mechanisms, we applied metabolomics, network pharmacology, and transcriptomics approaches.
RESULTS
The results demonstrated that CM extract effectively suppressed synovial inflammation in CIA rats, reducing joint degradation. CM's anti-inflammatory effects were mediated through the TNF signaling pathway, modulating glycerophospholipid and amino acid metabolism, including reduced levels of tryptophan, LysoPC, and asparagine. Molecular docking identified scutellarin and apigenin as key bioactive compounds. Additionally, immunofluorescence analysis revealed CM's therapeutic effects via TNF signaling inhibition and suppression of M1 macrophage polarization.
CONCLUSIONS
These findings highlight the therapeutic potential of CM for RA and support its development as a functional food or pharmaceutical product.
背景
葛根（CM）在治疗类风湿性关节炎（RA）和消化系统疾病方面显示出有前景的潜力，这归因于其富含多酚和黄酮类等生物活性化合物。尽管它常用于凉茶，但CM抗炎和关节保护作用的具体机制仍不清楚，限制了其作为功能性食品的开发。本研究调查了CM水提取物对胶原诱导性关节炎（CIA）大鼠RA的影响，并探索了其潜在机制。
方法
48只雌性Sprague-Dawley大鼠随机分为六组（每组 = 8只）：正常对照组、CIA模型组、甲氨蝶呤（MTX）组以及CM高、中、低剂量组。使用生化和组织学分析评估抗炎和关节保护作用。为阐明机制，我们应用了代谢组学、网络药理学和转录组学方法。
结果
结果表明，CM提取物有效抑制了CIA大鼠的滑膜炎症，减少了关节退化。CM的抗炎作用是通过TNF信号通路介导的，调节甘油磷脂和氨基酸代谢，包括色氨酸、溶血磷脂酰胆碱和天冬酰胺水平的降低。分子对接确定灯盏花素和芹菜素为关键生物活性化合物。此外，免疫荧光分析显示CM通过抑制TNF信号和抑制M1巨噬细胞极化发挥治疗作用。
结论
这些发现突出了CM对RA的治疗潜力，并支持其作为功能性食品或药品的开发。
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