用于农业塑料薄膜生物降解的微生物的筛选与评估
Selection and evaluation of microorganisms for biodegradation of agricultural plastic film.
作者信息
Zhang Jing, Chen Jing, Jia Ruimin, Dun Zhihen, Wang Baotong, Hu Xiaopin, Wang Yang
机构信息
1College of Plant Protection, Northwest A&F University, Yangling, Xianyang, 712100 Shaanxi China.
The Management Station for the Quality Construction of Cultivated Land in Gansu Province, Lanzhou, 313006 Gansu China.
出版信息
3 Biotech. 2018 Jul;8(7):308. doi: 10.1007/s13205-018-1329-5. Epub 2018 Jul 9.
Three isolates (HK1, GSDM02, and GSDM15) were tested for effectiveness in biodegradation of plastic films. Isolates were screened by plate on carbon-free medium and by using the clear-zone formation test. Their biodegradation ability was analyzed based on: film weight reduction, pH change of the fluid medium, a soil microbial biomass carbon test, scanning electron microscopy (SEM), and Fourier transform infrared spectrometry (FTIR). Polyvinyl alcohol (PVA) clear-zone and film weight reduction results revealed that the strain with a bigger clear-zone had a better biodegradation effect, that PVA can be evenly distributed in the medium, and that PVA can be a substitution for polyethylene in screening the biodegradation of strains. SEM and FTIR revealed that HK1 can tear the film apart and make surface chemical changes within 30 days. HK1 exhibited a better biodegradation effect in all tests, indicating its potential for helping solve the plastic pollution problems.
对三株分离菌（HK1、GSDM02和GSDM15）进行了塑料薄膜生物降解有效性测试。通过在无碳培养基上平板筛选和使用透明圈形成试验对分离菌进行筛选。基于以下方面分析它们的生物降解能力：薄膜重量减轻、流体培养基的pH变化、土壤微生物生物量碳测试、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）。聚乙烯醇（PVA）透明圈和薄膜重量减轻结果表明，透明圈较大的菌株具有更好的生物降解效果，PVA可在培养基中均匀分布，并且PVA可替代聚乙烯用于筛选菌株的生物降解。SEM和FTIR显示HK1可在30天内撕裂薄膜并使表面发生化学变化。HK1在所有测试中均表现出较好的生物降解效果，表明其在帮助解决塑料污染问题方面具有潜力。
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