Genetic Polymorphism Study on of Different Geographical Regions Based on DNA Barcoding.

is a very important vector for pathogens of many infectious diseases including dengue fever. In this study, we explored the genetic polymorphism of strains in different geographical regions using DNA barcoding of mitochondrial COI () gene. We collected sequence of 106 mosquitos from 6 provinces in China including Fujian, Guangdong, Hainan, Yunnan, and Taiwan. The length of the sequences is 709bp with the content of A+T (67.7%) greater than that of G+C (32.3%). We identified mutations in 90 (13.68%) loci, of which 57 (63.33%) are transitions, 28 (31.11%) are transversions, and 5 (5.56%) are hypervariable loci. In addition, we obtained 42 haplotypes, 4 (9.52%) of which are shared among different populations. The haplotype diversity of is 0.882 and nucleotide diversity is 0.01017. Moreover, the pedigree network diagram shows that most haplotypes are under parallel evolution, suggesting a local expansion of in history. Finally, the Neighbor-Joining tree of haplotypes reveals a certain correlation between haplotype clusters and geographical distribution, and there are differences among in different geographical regions. In conclusion, DNA barcoding of gene is an effective method to study the genetic structure of .