荧光寿命成像技术对牛心包胶原酶消化特性的无标记评估。
Label-Free Assessment of Collagenase Digestion on Bovine Pericardium Properties by Fluorescence Lifetime Imaging.
机构信息
Department of Materials Science Engineering, University of California, Davis, Davis, CA, USA.
Department of Biomedical Engineering, University of California, Davis, Davis, CA, USA.
出版信息
Ann Biomed Eng. 2018 Nov;46(11):1870-1881. doi: 10.1007/s10439-018-2087-6. Epub 2018 Jul 12.
The extracellular matrix architecture of bovine pericardium (BP) has distinct biochemical and biomechanical properties that make it a useful biomaterial in the field of regenerative medicine. Collagen represents the dominant structural protein of BP and is therefore intimately associated with the properties of this biomaterial. Enzymatic degradation of collagen molecules is critical for extracellular matrix turnover, remodeling and ultimately tissue regeneration. We present a quantitative, label-free and non-destructive method for monitoring changes in biochemical and biomechanical properties of BP during tissue degradation, based on multi-spectral fluorescence lifetime imaging (ms-FLIm). Strong correlations of fluorescence intensity ratio and average fluorescence lifetime were identified with collagen content, Young's Modulus and Ultimate tensile strength during collagenase degradation, indicating the potential of optically monitoring collagen degradation using ms-FLIm. The obtained results demonstrate the value of ms-FLIm to assess the quality of biomaterials in situ for applications in regenerative medicine.
牛心包的细胞外基质结构具有独特的生化和生物力学特性，使其成为再生医学领域有用的生物材料。胶原蛋白是牛心包的主要结构蛋白，因此与这种生物材料的特性密切相关。胶原分子的酶促降解对于细胞外基质的转化、重塑和最终的组织再生至关重要。我们提出了一种定量、无标记和非破坏性的方法，基于多光谱荧光寿命成像（ms-FLIm），用于监测组织降解过程中 BP 的生化和生物力学特性的变化。在胶原酶降解过程中，荧光强度比和平均荧光寿命与胶原蛋白含量、杨氏模量和极限拉伸强度之间存在很强的相关性，这表明使用 ms-FLIm 进行光监测胶原蛋白降解具有潜力。所得结果表明，ms-FLIm 可用于评估再生医学应用中生物材料的原位质量。
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