Department of Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Johann Wolfgang Goethe University, Max-von-Laue Str. 9, D-60438, Frankfurt, Germany.
Göttingen Genomics Laboratory, Institute for Microbiology and Genetics, Georg August University, Grisebachstr. 8, D-37077, Göttingen, Germany.
Environ Microbiol. 2018 Dec;20(12):4369-4384. doi: 10.1111/1462-2920.14356. Epub 2018 Oct 24.
Methanol derived from plant tissue is ubiquitous in anaerobic sediments and a good substrate for anaerobes growing on C compounds such as methanogens and acetogens. In contrast to methanogens little is known about the physiology, biochemistry and bioenergetics of methanol utilization in acetogenic bacteria. To fill this gap, we have used the model acetogen Acetobacterium woodii to study methanol metabolism using physiological and biochemical experiments paired with molecular studies and transcriptome analysis. These studies identified the genes and enzymes involved in acetogenesis from methanol and the redox carriers involved. We will present the first comprehensive model for carbon and electron flow from methanol in an acetogen and the bioenergetics of acetogenesis from methanol.
植物组织衍生的甲醇在厌氧沉积物中无处不在，是厌氧微生物利用 C 化合物（如产甲烷菌和产乙酸菌）生长的良好底物。与产甲烷菌相比，人们对产乙酸菌利用甲醇的生理学、生物化学和生物能量学知之甚少。为了填补这一空白，我们使用模型产乙酸菌 A. woodii 来研究甲醇代谢，结合生理生化实验、分子研究和转录组分析。这些研究确定了参与甲醇产乙酸的基因和酶以及涉及的氧化还原载体。我们将提出第一个关于产乙酸菌中甲醇的碳和电子流以及甲醇产乙酸的生物能量学的综合模型。
