Chen Yonggang, Wang Shuanke, Geng Bin, Yi Zhigang
Department of Orthopaedics, Lanzhou University Second Hospital, Lanzhou, Gansu 730030, China.
J BUON. 2018 May-Jun;23(3):735-740.
Osteosarcoma is the most common type of primary malignancy of bone in children and adults. The treatment options for osteosarcoma are limited and are associated with a number of drawbacks. Therefore there is an urgent need to look for more efficient options for the treatment of this disease. Flavonoids have been considered as important anticancer agents due to their efficacy and lower cytotoxicity. In the present study we evaluated the anticancer effects of pelargonidin in U2OS osteosarcoma cell line.
Cell viability was assessed by MTT assay. Reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and cell cycle analysis was done by flow cytometry. Expression of proteins was examined by western blotting.
Pelargonidin exhibited significant anticancer effects on osteosarcoma U2OS cell line with an IC50 of 15 μM. The anticancer effects of pelargonidin were due to induction of autophagy as evidenced from the expression of LC3-I, LC-3II and Beclin-1. Moreover, pelargonidin triggered ROSinduced reduction in MMP and triggered G2/M cell cycle arrest. In addition, pelargonidin inhibited the expression of p-PI3K and p-AKT in a concentration-dependent manner.
Taken together, these results indicated that pelargonidin may prove a potential lead drug for the treatment of osteosarcoma.
骨肉瘤是儿童和成人中最常见的原发性骨恶性肿瘤类型。骨肉瘤的治疗选择有限且存在诸多弊端。因此，迫切需要寻找更有效的治疗该疾病的方法。黄酮类化合物因其功效和较低的细胞毒性而被视为重要的抗癌剂。在本研究中，我们评估了天竺葵素对U2OS骨肉瘤细胞系的抗癌作用。
通过MTT法评估细胞活力。通过流式细胞术进行活性氧（ROS）、线粒体膜电位（MMP）和细胞周期分析。通过蛋白质印迹法检测蛋白质表达。
天竺葵素对骨肉瘤U2OS细胞系表现出显著的抗癌作用，IC50为15 μM。天竺葵素的抗癌作用归因于自噬的诱导，这从LC3-I、LC-3II和Beclin-1的表达中得到证实。此外，天竺葵素引发ROS诱导的MMP降低并引发G2/M细胞周期阻滞。此外，天竺葵素以浓度依赖性方式抑制p-PI3K和p-AKT的表达。
综上所述，这些结果表明天竺葵素可能是治疗骨肉瘤的一种潜在先导药物。
