Tang Zhiling
Department of Urology, Jiaxing Second Hospital, Zhejiang, China.
J BUON. 2018 May-Jun;23(3):782-786.
To investigate the effects of human enhancer of filamentation 1 (HEF1) gene on the proliferation, invasion and metastasis of bladder cancer cells.
Three human bladder cancer cell lines (T24, EJ and BIU-87) were selected to extract total RNA at logarithmic growth phase. The relative expression level of HEF1 in these cell lines was detected by semi-quantitative reverse transcription PCR (RT-PCR), and the cell lines with relatively high expression and relatively low expression level of HEF1 cells were identified. HEF1 overexpression recombinant adenovirus was transfected into the bladder cancer cells with low expression level of HEF1, and HEF1 siRNA was transfected into the bladder cancer cells with high expression level of HEF1. MTT assay, migration assay and invasion assay were performed to detect the proliferation, migration and invasion of bladder cancer cells.
The relative expression level of HEF1 mRNA in T24 cell line was significantly higher than that in EJ and BIU-87 cells, and BIU-87 cell line showed the lowest expression level (p<0.05). After transfection with HEF1 overexpression recombinant adenovirus, the proliferation, migration and invasion of BIU-87 cells were significantly improved (p<0.05). After siRNA silencing, the proliferation, migration and invasion of T24 cells were significantly inhibited (p<0.05).
High expression level of HEF1 gene can promote the proliferation, invasion and metastasis of bladder cancer cells.
探讨人丝状化增强因子1（HEF1）基因对膀胱癌细胞增殖、侵袭和转移的影响。
选取三株人膀胱癌细胞系（T24、EJ和BIU-87），在对数生长期提取总RNA。采用半定量逆转录聚合酶链反应（RT-PCR）检测这些细胞系中HEF1的相对表达水平，鉴定出HEF1表达相对较高和相对较低的细胞系。将HEF1过表达重组腺病毒转染至HEF1表达水平低的膀胱癌细胞中，将HEF1小干扰RNA（siRNA）转染至HEF1表达水平高的膀胱癌细胞中。采用MTT法、迁移实验和侵袭实验检测膀胱癌细胞的增殖、迁移和侵袭能力。
T24细胞系中HEF1 mRNA的相对表达水平显著高于EJ和BIU-87细胞，BIU-87细胞系表达水平最低（p<0.05）。转染HEF1过表达重组腺病毒后，BIU-87细胞的增殖、迁移和侵袭能力显著提高（p<0.05）。siRNA沉默后，T24细胞的增殖、迁移和侵袭能力显著受到抑制（p<0.05）。
HEF1基因高表达可促进膀胱癌细胞的增殖,侵袭和转移。
